LOCUS       sequence-151972-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..132
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             151..525
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    655..776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(692..711)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     746..765
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
       61 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
      121 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
      181 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
      241 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
      301 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
      361 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
      421 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
      481 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
      541 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
      601 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
      661 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      721 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      781 gtctgaatt
//
