LOCUS       sequence-151974-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry 
            marker in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, 
            Elowitz MB
  TITLE     Dynamics of epigenetic regulation at the single-cell level.
  JOURNAL   Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
  PUBMED    26912859
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "720-4"
COMMENT     Sequence Label: pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        89..136
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             155..529
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    659..780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(696..715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     750..769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
       61 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
      121 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
      181 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
      241 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
      301 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
      361 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
      421 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
      481 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
      541 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
      601 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
      661 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      721 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      781 tcatgtctga atttc
//
