LOCUS       sequence-151981-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Bait Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pPA-RL-GW
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..234
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             235..408
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 cttaagcttg gtaccgagct cggatccgcc gccaccatgg ttgcgcaaca cgatgaagcc
       61 gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct
      121 aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa
      181 agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac
      241 aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta
      301 aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct
      361 aaccttttag cagaagctaa aaagctaaat ggtgctcagg cgccgaaagt agacgcgaat
      421 tcgatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac tgggcctcag
      481 tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta ctatgattcc
      541 gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc cagctacctg
      601 tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc tgatctgatc
      661 ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga tcactacaag
      721 tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt tgtgggccac
      781 gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa gatcaaggcc
      841 atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg gcctgacatc
      901 gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct tgagaataac
      961 ttcttcgtcg agaccatgct cccaagcaag
//
