LOCUS       sequence-151984-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Bait Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pPA-RL-GW
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..147
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(188..493)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(290..309)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cctctagagc ggccgcgata tcaccacttt gtacaagaaa gctgaacgag aaacgtaaaa
       61 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      121 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
      181 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
      241 ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
      301 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggagactt
      361 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
      421 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
      481 ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt catttcaata
      541 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga
      601 cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg
      661 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat
      721 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
      781 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac gcgtttacgc
      841 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      901 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      961 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1021 taaatcaaaa ctggtgaaac tcacccaggg attggctga
//
