LOCUS       sequence-151996-        8432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DULIP Bait Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pPA-RL-GW
FEATURES             Location/Qualifiers
     source          1..8432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      938..947
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             968..1141
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1142..1315
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             1331..2263
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    2276..2400
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2425..2430,2431..2448,2449..2455)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2509..3168
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2575..2594)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3510..3815
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3694..3713
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3856..3980)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4026..4043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4032..4256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4302..4730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4389..4408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4599..4620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4739..4759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4744..5073
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4924..5059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4986..5005
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5140..5934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5194..5213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5804..5823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6108..6229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6145..6164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6199..6218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6278..6294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6278..6294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6291..6313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6302..6318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6326..6332,6333..6350,6351..6356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6371..6392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6509..6526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6680..7265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6760..6779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7436..8227,8228..8296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8059..8078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8297..8401)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccgcc accatggttg cgcaacacga
      961 tgaagccgtg gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca
     1021 tttacctaac ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc
     1081 aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa
     1141 agtagacaac aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc
     1201 taacttaaac gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca
     1261 aagcgctaac cttttagcag aagctaaaaa gctaaatggt gctcaggcgc cgaaagtaga
     1321 cgcgaattcg atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg
     1381 gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta
     1441 tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag
     1501 ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga
     1561 tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca
     1621 ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt
     1681 gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat
     1741 caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc
     1801 tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga
     1861 gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga
     1921 ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct
     1981 ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt
     2041 ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc
     2101 cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga
     2161 gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa
     2221 gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagctcgagg atatcacaag
     2281 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     2341 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactttg
     2401 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     2461 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     2521 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     2581 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     2641 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     2701 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     2761 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     2821 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     2881 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     2941 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3001 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3061 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     3121 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc
     3181 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     3241 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     3301 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     3361 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     3421 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     3481 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     3541 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     3601 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     3661 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     3721 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3781 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc
     3841 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3901 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3961 gctttcttgt acaaagtggt gatatcgcgg ccgctctaga gggcccgttt aaacccgctg
     4021 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4081 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4141 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4201 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4261 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4501 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4621 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4681 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4741 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4801 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4861 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4921 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4981 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5041 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5101 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5161 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5221 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5281 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5341 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5401 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5461 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5521 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5581 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5641 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5701 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5761 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5821 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5881 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5941 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     6001 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6061 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6241 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8401 gcacatttcc ccgaaaagtg ccacctgacg tc
//
