LOCUS       sequence-152007-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Prey Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pFL-V5-GW
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(103..121)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cttaagcttg gtaccgagct cggatccgcc gccaccatgg aagacgccaa aaacataaag
       61 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
      121 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
      181 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
      241 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
      301 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
      361 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
      421 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
      481 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
      541 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
      601 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
      661 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
      721 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
      781 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
      841 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
      901 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
      961 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga a
//
