LOCUS       sequence-152011-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Prey Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pFL-V5-GW
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..144
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(185..490)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(287..306)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctagagcggc cgcgatatca ccactttgta caagaaagct gaacgagaaa cgtaaaatga
       61 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      121 aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg
      181 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
      241 tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca
      301 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
      361 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
      421 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
      481 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
      541 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
      601 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
      661 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
      721 tctttttgac atacttcggg tatacatatc agtatatatt cttataccgc aaaaatcagc
      781 gcgcaaatac gcatactgtt atctggcttt tagtaagccg gatccacgcg tttacgcccc
      841 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      901 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      961 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1021 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     1081 ccctttaggg aaatag
//
