LOCUS       sequence-152015-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Prey Destination Vector (Gateway, C-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pGW-FL-V5
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(147..152,153..170,171..177)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             231..890
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(297..316)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
       61 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      121 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      181 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      241 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      301 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      361 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      421 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      481 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      541 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      601 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      661 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      721 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
      781 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
      841 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tctagaggat
      901 ccggcttact aaaagcc
//
