LOCUS       sequence-152048-        6711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary DEST vector for plant expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  TITLE     pAM-PAT-ProUBQ10 GW
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAM-PAT-ProATE1 GW
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    348..372
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      484..1193
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1254..1358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1359..1427,1428..2219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1577..1596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2390..2978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2879..2898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3290..3314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3408..3660)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3680..4231)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4274..4453)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    4984..5108
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5133..5138,5139..5156,5157..5163)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5217..5876
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5283..5302)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6218..6523
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6402..6421
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6564..6688)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 agcttgttga gaattcatcg cccgggactg ggttaacttc cgatcgattc tagactagtt
       61 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
      121 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
      181 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
      241 taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac cgcggtggag
      301 ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
      361 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      421 tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc aaggcaagac
      481 cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
      541 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cggccgccgg
      601 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
      661 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
      721 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
      781 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
      841 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
      901 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
      961 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     1021 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     1081 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     1141 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
     1201 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgacaagcta
     1261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2521 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3001 tacggttcct ggccttttgc tggccttttg ctcacatgga ctctagctag aggatcacag
     3061 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     3121 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     3181 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     3241 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     3301 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
     3361 tgatcgaata ctaacgtctc taccagatat cagcttgcat gccggtcgat ctagtaacat
     3421 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     3481 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     3541 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     3601 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     3661 tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg ggcggcaccg
     3721 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
     3781 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
     3841 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
     3901 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
     3961 cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga
     4021 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
     4081 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
     4141 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
     4201 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc ccctcgatcg agttgagagt
     4261 gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt
     4321 gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg
     4381 tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc
     4441 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     4501 tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcggt
     4561 ttgggcgcgc cgtttgacca gaactctacg cctccgtagt cttcggaatt gttgttctga
     4621 ccgccgatta ctgctcgcca caggcttgcc atcttccgaa gattgaactg gagaattagg
     4681 gttttgatcc aaatctgccg atgctatcga aaatctgttt atcgattcca ttattttttc
     4741 gtgtttatct tttgccccaa aatgttttta tttttcattt tcttccctta aaatatttat
     4801 aattacataa caaccccttc attttgccaa ttgcataaca gtcagcaaca atgttttaat
     4861 attgaaaaaa aaagtcaaat gtgaaagaag attatgtctt cttctccaaa cggaaagagc
     4921 ttagcaaaat tcttctgaat gaataatcgg cgattctcga gaaagaggat ccacctgagg
     4981 atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     5041 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     5101 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     5161 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     5221 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     5281 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     5341 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     5401 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     5461 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     5521 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     5581 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     5641 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     5701 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     5761 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     5821 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     5881 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     5941 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     6001 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     6061 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     6121 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     6181 aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta
     6241 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     6301 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     6361 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     6421 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     6481 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     6541 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     6601 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     6661 ctcgttcagc tttcttgtac aaagtggtga tcctccgttc catgggctag a
//
