LOCUS       sequence-152064-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM
  TITLE     SOX9 inhibits beta-TrCP-mediated protein degradation to promote 
            nuclear GLI1 expression and cancer stem cell properties.
  JOURNAL   J Cell Sci. 2015 Jan 27. pii: jcs.162164.
  PUBMED    25632159
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jan 27. pii: jcs.162164."
COMMENT     Sequence Label: pLUT7 HA-GLI1
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..90
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        71..89
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg taatacgact cactataggg tgctagcacc tagagacgcc atgtatcctt
      121 atgacgtgcc tgactatgcc agcctgggag gacctttcaa ctcgatgacc ccaccaccaa
      181 tcagtagcta tggcgagccc tgctgtctcc ggcccctccc cagtcagggg gcccccagtg
      241 tggggacaga aggactgtct ggcccgccct tctgccacca agctaacctc atgtccggcc
      301 cccacagtta tgggccagcc agagagacca acagctgcac cgagggccca ctcttttctt
      361 ctccccggag tgcagtcaag ttgaccaaga agcgggcact gtccatctca cctctgtcgg
      421 atgccagcct ggacctgcag acggttatcc gcacctcacc cagctccctc gtagctttca
      481 tcaactcgcg atgcacatct ccaggaggct cctacggtca tctctccatt ggcaccatga
      541 gcccatctct gggattccca gcccagatga atcaccaaaa agggccctcg ccttcctttg
      601 gggtccagcc ttgtggtccc catgactctg cccggggtgg gatgatccca catcctcagt
      661 cccggggacc cttcccaact tgccagctga agtctgagct ggacatgctg gttggcaagt
      721 gccgggagga acccttggaa ggtgatatgt ccagccccaa ctccacaggc atacaggatc
      781 ccctgttggg gatgctggat gggcgggagg acctcgagag aga
//
