LOCUS       sequence-152066-        9913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged Gli1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM
  TITLE     SOX9 inhibits beta-TrCP-mediated protein degradation to promote 
            nuclear GLI1 expression and cancer stem cell properties.
  JOURNAL   J Cell Sci. 2015 Jan 27. pii: jcs.162164.
  PUBMED    25632159
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2015 Jan 27. pii: jcs.162164."
COMMENT     Sequence Label: pLUT7 HA-GLI1
FEATURES             Location/Qualifiers
     source          1..9913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             85..129
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    396..512
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        537..840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        841..1044
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     995..1015
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1041..1065
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1113..1132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1113..1131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1156..1182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1162..1188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     misc_feature    4565..5153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4618..4638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5036..5047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5244..5477
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5549..5670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5586..5605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5640..5659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5710..5845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5877..5893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5877..5893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5890..5912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5901..5917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5925..5931,5932..5949,5950..5955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6108..6125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6279..6867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6359..6378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7038..7829,7830..7898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7661..7680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7899..8003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8071..8089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8127..8149)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8249..8268
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8462..8484
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8476..8493
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8477..8493
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8508..8734
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8735..8915
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8962..9087
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9580..9813
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
       61 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
      121 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
      181 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
      241 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
      301 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
      361 cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg
      421 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      481 ttacaaaaac aaattacaaa attcaaaatt ttatcgataa actagtggga gttccgcgtt
      541 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      601 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      661 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      721 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      781 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      841 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      901 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      961 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1021 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     1081 ccacgctgtt ttgacctcca tagaagacac cgtaatacga ctcactatag ggtgctagca
     1141 cctagagacg ccatgtatcc ttatgacgtg cctgactatg ccagcctggg aggacctttc
     1201 aactcgatga ccccaccacc aatcagtagc tatggcgagc cctgctgtct ccggcccctc
     1261 cccagtcagg gggcccccag tgtggggaca gaaggactgt ctggcccgcc cttctgccac
     1321 caagctaacc tcatgtccgg cccccacagt tatgggccag ccagagagac caacagctgc
     1381 accgagggcc cactcttttc ttctccccgg agtgcagtca agttgaccaa gaagcgggca
     1441 ctgtccatct cacctctgtc ggatgccagc ctggacctgc agacggttat ccgcacctca
     1501 cccagctccc tcgtagcttt catcaactcg cgatgcacat ctccaggagg ctcctacggt
     1561 catctctcca ttggcaccat gagcccatct ctgggattcc cagcccagat gaatcaccaa
     1621 aaagggccct cgccttcctt tggggtccag ccttgtggtc cccatgactc tgcccggggt
     1681 gggatgatcc cacatcctca gtcccgggga cccttcccaa cttgccagct gaagtctgag
     1741 ctggacatgc tggttggcaa gtgccgggag gaacccttgg aaggtgatat gtccagcccc
     1801 aactccacag gcatacagga tcccctgttg gggatgctgg atgggcggga ggacctcgag
     1861 agagaggaga agcgtgagcc tgaatctgtg tatgaaactg actgccgttg ggatggctgc
     1921 agccaggaat ttgactccca agagcagctg gtgcaccaca tcaacagcga gcacatccac
     1981 ggggagcgga aggagttcgt gtgccactgg gggggctgct ccagggagct gaggcccttc
     2041 aaagcccagt acatgctggt ggttcacatg cgcagacaca ctggcgagaa gccacacaag
     2101 tgcacgtttg aagggtgccg gaagtcatac tcacgcctcg aaaacctgaa gacgcacctg
     2161 cggtcacaca cgggtgagaa gccatacatg tgtgagcacg agggctgcag taaagccttc
     2221 agcaatgcca gtgaccgagc caagcaccag aatcggaccc attccaatga gaagccgtat
     2281 gtatgtaagc tccctggctg caccaaacgc tatacagatc ctagctcgct gcgaaaacat
     2341 gtcaagacag tgcatggtcc tgacgcccat gtgaccaaac ggcaccgtgg ggatggcccc
     2401 ctgcctcggg caccatccat ttctacagtg gagcccaaga gggagcggga aggaggtccc
     2461 atcagggagg aaagcagact gactgtgcca gagggtgcca tgaagccaca gccaagccct
     2521 ggggcccagt catcctgcag cagtgaccac tccccggcag ggagtgcagc caatacagac
     2581 agtggtgtgg aaatgactgg caatgcaggg ggcagcactg aagacctctc cagcttggac
     2641 gagggacctt gcattgctgg cactggtctg tccactcttc gccgccttga gaacctcagg
     2701 ctggaccagc tacatcaact ccggccaata gggacccggg gtctcaaact gcccagcttg
     2761 tcccacaccg gtaccactgt gtcccgccgc gtgggccccc cagtctctct tgaacgccgc
     2821 agcagcagct ccagcagcat cagctctgcc tatactgtca gccgccgctc ctccctggcc
     2881 tctcctttcc cccctggctc cccaccagag aatggagcat cctccctgcc tggccttatg
     2941 cctgcccagc actacctgct tcgggcaaga tatgcttcag ccagaggggg tggtacttcg
     3001 cccactgcag catccagcct ggatcggata ggtggtcttc ccatgcctcc ttggagaagc
     3061 cgagccgagt atccaggata caaccccaat gcaggggtca cccggagggc cagtgaccca
     3121 gcccaggctg ctgaccgtcc tgctccagct agagtccaga ggttcaagag cctgggctgt
     3181 gtccataccc cacccactgt ggcaggggga ggacagaact ttgatcctta cctcccaacc
     3241 tctgtctact caccacagcc ccccagcatc actgagaatg ctgccatgga tgctagaggg
     3301 ctacaggaag agccagaagt tgggacctcc atggtgggca gtggtctgaa cccctatatg
     3361 gacttcccac ctactgatac tctgggatat gggggacctg aaggggcagc agctgagcct
     3421 tatggagcga ggggtccagg ctctctgcct cttgggcctg gtccacccac caactatggc
     3481 cccaacccct gtccccagca ggcctcatat cctgacccca cccaagaaac atggggtgag
     3541 ttcccttccc actctgggct gtacccaggc cccaaggctc taggtggaac ctacagccag
     3601 tgtcctcgac ttgaacatta tggacaagtg caagtcaagc cagaacaggg gtgcccagtg
     3661 gggtctgact ccacaggact ggcaccctgc ctcaatgccc accccagtga ggggccccca
     3721 catccacagc ctctcttttc ccattacccc cagccctctc ctccccaata tctccagtca
     3781 ggcccctata cccagccacc ccctgattat cttccttcag aacccaggcc ttgcctggac
     3841 tttgattccc ccacccattc cacagggcag ctcaaggctc agcttgtgtg taattatgtt
     3901 caatctcaac aggagctact gtgggagggt gggggcaggg aagatgcccc cgcccaggaa
     3961 ccttcctacc agagtcccaa gtttctgggg ggttcccagg ttagcccaag ccgtgctaaa
     4021 gctccagtga acacatatgg acctggcttt ggacccaact tgcccaatca caagtcaggt
     4081 tcctatccca ccccttcacc atgccatgaa aattttgtag tgggggcaaa tagggcttca
     4141 catagggcag cagcaccacc tcgacttctg cccccattgc ccacttgcta tgggcctctc
     4201 aaagtgggag gcacaaaccc cagctgtggt catcctgagg tgggcaggct aggagggggt
     4261 cctgccttgt accctccccc cgaaggacag gtatgtaacc ccctggactc tcttgatctt
     4321 gacaacactc agctggactt tgtggctatt ctggatgagc cccaggggct gagtcctcct
     4381 ccttcccatg atcagcgggg cagctctgga cataccccac ctccctctgg gccccccaac
     4441 atggctgtgg gcaacatgag tgtcttactg agatccctac ctggggaaac agaattcctc
     4501 aactctagtg cctaagatcc cccgggctgc aggaattcga tatcaagcta attctagagt
     4561 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4621 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4681 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4741 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4801 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4861 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4921 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4981 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5041 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5101 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5161 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5221 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5281 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5341 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5401 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5461 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     5521 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     5581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5641 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5761 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5821 tagtgaggag gcttttttgg aggcctaggc ttttgcgggc ccaaattcgt aatcatgtca
     5881 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5941 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8041 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     8101 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8161 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8221 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     8281 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     8341 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8401 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8461 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc
     8521 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8581 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8641 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8701 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8881 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8941 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     9001 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     9061 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     9121 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     9181 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9241 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9301 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9361 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9421 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9481 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9541 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9601 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9661 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9721 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9781 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9841 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9901 ttggaatcac acg
//
