LOCUS       sequence-152092-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent calcium sensor protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G,
            Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens 
            MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS
  TITLE     Sensitive red protein calcium indicators for imaging neural 
            activity.
  JOURNAL   eLife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727.
  PUBMED    27011354
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2016-03-24"; volume: "5. pii: e12727. doi"; pages: " 
            10.7554/eLife.12727"
COMMENT     Sequence Label: pGP-CMV-NES-jRCaMP1a
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(169..177,178..192)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             220..276
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 gaaccgtcag atccgctagc gctaccggac tcagatctcg ccaccatgct gcagaacgag
       61 cttgctctta agttggctgg acttgatatt aacaagactg gaggaggttc tcatcatcat
      121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      181 gatgacgata aggatctcgc aacaatggtc gactcatcac gtcgtaagtg gaataagaca
      241 ggtcacgcag tcagagctat aggtcggctg agctcagcga tcaactgcga aatgatgtac
      301 ccagcggatg gtggtctgcg tggttacact cacatggcgc tgaaagttga tggcggcggt
      361 cacctgtcct gttctttcgt gaccacctac cgctccaaaa agactgtcgg caacattaag
      421 atgcctgcca ttcatagcgt cagccaccgt ctggagcgcc tggaggagag cgataacgaa
      481 atgtttgtcg tacagcgtga acacgcagtt gccaagtttg tgggcctggg tggtggcggc
      541 ggtaccggag ggagcatgaa ctccctgatc aaggagaaca tgcgtatgaa agtggttctg
      601 gaaggctccg taaacggcca ccagttcaaa tgcactggtg aaggcgaagg caacccgtat
      661 atgggcaccc agactatgcg tatcaaagtg atcgagggtg gtccgctgcc gtttgcgttc
      721 gacatcctgg cgacgtcctt tatgtatggc tcccgtacct tcatcaaata tccgaaaggc
      781 atcccggatt tctttaagca gtccttcccg gaaggtttta cctgggaacg tgtgacccgt
      841 tacgaagacg gcggcgtaat taccgttatg caagacacgt ctctggagga tggctgcctg
      901 gtgtatcacg tgcaggttcg cggtgtgaac tt
//
