LOCUS       sequence-152113-        5863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Mani SA, Yang J, Brooks M, Schwaninger G, Zhou A, Miura N, Kutok JL,
            Hartwell K, Richardson AL, Weinberg RA
  TITLE     Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is 
            associated with aggressive basal-like breast cancers.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jun 12. 104(24):10069-74.
  PUBMED    17537911
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Jun 12. 104(24):10069-74."
COMMENT     Sequence Label: pBabe puro Snail
FEATURES             Location/Qualifiers
     source          1..5863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             68..537
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(758..1357)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(841..861)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1338..1357
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1367..1696)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1381..1516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1681..1701
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(2536..2952)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2552..2568)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2584..2606)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3153..3352
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3417..3886
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3917..4021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4022..4090,4091..4882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4240..4259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5042..5630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5531..5550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5784..5801
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caactatggc catttaatgt aaatacttaa gaaaaaaaac caaattaatt ttgatacatg
       61 ctgcatgtga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
      121 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
      181 gggcgactca gtcaatcgga ggactggcgc cccgagtgag gggttgtggg ctcttttatt
      241 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
      301 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
      361 gaaactgctg agggctggac cgcatctggg gaccatctgt tcttggccct gagccggggc
      421 aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca tctgttcttg
      481 gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcagctg
      541 ttccatctgt tcctgacctt gatctgaact tctctattct cagttatgta tttttccatg
      601 ccttgcaaaa tggcgttact taagctagct tgccaaacct acaggtgggg tctttcattc
      661 cccccttttt ctggagacta aataaaatct tttattttat cgatgcatgg ggtcgtgcgc
      721 tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca
      781 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
      841 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
      901 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
      961 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     1021 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     1081 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     1141 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     1201 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     1261 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     1321 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctttttg caaaagccta
     1381 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     1441 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     1501 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     1561 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     1621 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     1681 accctaactg acacacattc cacagggtcg accactgtgc tggcgaattc tcagcgggga
     1741 catcctgagc agccggactc ttggtgcttg tggagcaggg acattcggga gaaggtccga
     1801 gcacacgcct ggcactggta cttcttgaca tctgagtggg tctggaggtg ggcccgcagg
     1861 ttggagcggt cagcgaaggc acggctgcag tggggacagg agaagggctt ctcgccagtg
     1921 tgggtccgga catggccttg tagcagccag ggcctagaga aggccttccc gcaggttccg
     1981 cagacgcagg gcagcgtgtg gcttcggatg tgcatcttga gggcacccag gctgaggtat
     2041 tccttgttgc agtatttgca gttgaaggcc tttcgagcct ggagatcctt ggcctcagag
     2101 agctgggcca gctgcttggg cacttggccc aagcctggga aggcagcata ggcctcggcc
     2161 tccaaggaag agactgaagt agaggagaag gacgaaggag ccggtgaggg tgggctgggg
     2221 ggctgggagc ctttcccact gtcctcatct gacagggagg tcagctctgc caccctggga
     2281 ctctcctgga gccgaaggga ggcccaggca attggctggg cttggggcgc caggacagag
     2341 tcccagatga gcattggcag cgaggcggtg gggttgagga tctccggagg tgggatggct
     2401 gccagcaggt gggcctggtc gtagggctgc tggaaggtaa actctggatt agagtcctgc
     2461 agctcgctgt agttaggctt ccgattgggg tcggagggct tcctgacgag gaaagagcgc
     2521 ggcatggatc cggccggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg
     2581 cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg
     2641 aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca
     2701 ggtcacgatg taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat
     2761 cttaacctgg gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa
     2821 ggttggccat tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg
     2881 gttgtgagcg atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt
     2941 aacagtctgg ccctaatttt cagacaaata cagaaacaca gtcagacaga gacaacacag
     3001 aacgatgctg cagcgctgca gcagacaaga cgcgcggctt cggttccaaa ccgaaagcaa
     3061 aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc
     3121 tgacggggtc ggattccaca tcgactccct tcctcaggtc gggccacaaa aacggccccc
     3181 aaagtccctg ggacgtctcc cagggttgcg gccgggtgtt cagaactcgt cagttccacc
     3241 acgggtccgc cagatacaga gctagttagc taactagtac cgacgcaggc gcataaaatc
     3301 agtcatagac actagacaat cggacagaca cagataagtt gctggccagc ttgcctcccg
     3361 gtggtgggtc ggtggtccct gggcaggggt ctcccgatcc cggacgagcc cccaaatgaa
     3421 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     3481 cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag
     3541 tcaatcggag gactggcgcc ccgagtgagg ggttgtgggc tcttttattg agctcgggga
     3601 gcagaagcgc gcgaacagaa gcgagaagcg aactgattgg ttagttcaaa taaggcacag
     3661 ggtcatttca ggtccttggg gcaccctgga aacatctgat ggttctctag aaactgctga
     3721 gggctggacc gcatctgggg accatctgtt cttggccctg agccggggca ggaactgctt
     3781 accacagata tcctgtttgg cccatattca gctgttccat ctgttcttgg ccctgagccg
     3841 gggcaggaac tgcttaccac agatatcctg tttggcccat attcaggctg caggtggcac
     3901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4021 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4081 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4141 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4201 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4261 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4321 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4381 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4441 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4501 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4561 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4621 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4681 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4741 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4801 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4861 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4921 tttgcggccg caaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5161 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5701 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5761 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5821 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atg
//
