LOCUS       sequence-152116-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Dower K, Rosbash M
  TITLE     T7 RNA polymerase-directed transcripts are processed in yeast and 
            link 3' end formation to mRNA nuclear export.
  JOURNAL   RNA. 2002 May;8(5):686-97.
  PUBMED    12022234
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2002-05"; volume: "8(5)"; pages: "686-97"
COMMENT     Sequence Label: pRS314-T7-GFP
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(67..85)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(118..136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(143..160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(143..159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      300..755
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(387..406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     597..618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcttctcct ttgctagcca tggatccttt gtttgtttat gtgtgtttat tcgaaactaa
       61 gttctcccta tagtgagtcg tattagcggc cgccaccgcg gtggagctcc aattcgccct
      121 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      241 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
      301 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      361 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      421 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      481 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      541 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      601 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      661 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      721 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
      781 catctgtgcg gtatttcaca ccgcatagat cggcaagtgc acaaacaata cttaaataaa
      841 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
      901 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
      961 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacata
//
