LOCUS       sequence-152137-        7567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, 
            Guarente L, Weinberg RA
  TITLE     hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
  JOURNAL   Cell 2001 Oct 19;107(2):149-59.
  PUBMED    11672523
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2001-10-19"; volume: "107(2)"; pages: "149-59"
COMMENT     Sequence Label: pYESir2-puro
FEATURES             Location/Qualifiers
     source          1..7567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1926..2395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2460..2659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2860..3276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3206..3228
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3244..3260
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3290..5533
                     /codon_start=1
                     /product="human NAD+-dependent protein deacetylase 
                     sirtuin-1"
                     /label=SIRT1
                     /note="homolog of budding yeast Sir2"
                     /translation="MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLE
                     RSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNG
                     PGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESD
                     EEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQ
                     IVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYA
                     RLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLL
                     RNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCP
                     ADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHE
                     VPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRT
                     QKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCME
                     EKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISR
                     RLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESE
                     IEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS"
     primer_bind     complement(5608..5627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5609..5627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5634..5651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5634..5650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5643..5665)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5815..5835)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5820..6149
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6000..6135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6159..6758
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6159..6178)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6655..6675
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6979..7448
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatccacca tggcggacga
     3301 ggcggccctc gcccttcagc ccggcggctc cccctcggcg gcgggggccg acagggaggc
     3361 cgcgtcgtcc cccgccgggg agccgctccg caagaggccg cggagagatg gtcccggcct
     3421 cgagcggagc ccgggcgagc ccggtggggc ggccccagag cgtgaggtgc cggcggcggc
     3481 caggggctgc ccgggtgcgg cggcggcggc gctgtggcgg gaggcggagg cagaggcggc
     3541 ggcggcaggc ggggagcaag aggcccaggc gactgcggcg gctggggaag gagacaatgg
     3601 gccgggcctg cagggcccat ctcgggagcc accgctggcc gacaacttgt acgacgaaga
     3661 cgacgacgac gagggcgagg aggaggaaga ggcggcggcg gcggcgattg ggtaccgaga
     3721 taaccttctg ttcggtgatg aaattatcac taatggtttt cattcctgtg aaagtgatga
     3781 ggaggataga gcctcacatg caagctctag tgactggact ccaaggccac ggataggtcc
     3841 atatactttt gttcagcaac atcttatgat tggcacagat cctcgaacaa ttcttaaaga
     3901 tttattgccg gaaacaatac ctccacctga gttggatgat atgacactgt ggcagattgt
     3961 tattaatatc ctttcagaac caccaaaaag gaaaaaaaga aaagatatta atacaattga
     4021 agatgctgtg aaattactgc aagagtgcaa aaaaattata gttctaactg gagctggggt
     4081 gtctgtttca tgtggaatac ctgacttcag gtcaagggat ggtatttatg ctcgccttgc
     4141 tgtagacttc ccagatcttc cagatcctca agcgatgttt gatattgaat atttcagaaa
     4201 agatccaaga ccattcttca agtttgcaaa ggaaatatat cctggacaat tccagccatc
     4261 tctctgtcac aaattcatag ccttgtcaga taaggaagga aaactacttc gcaactatac
     4321 ccagaacata gacacgctgg aacaggttgc gggaatccaa aggataattc agtgtcatgg
     4381 ttcctttgca acagcatctt gcctgatttg taaatacaaa gttgactgtg aagctgtacg
     4441 aggagatatt tttaatcagg tagttcctcg atgtcctagg tgcccagctg atgaaccgct
     4501 tgctatcatg aaaccagaga ttgtgttttt tggtgaaaat ttaccagaac agtttcatag
     4561 agccatgaag tatgacaaag atgaagttga cctcctcatt gttattgggt cttccctcaa
     4621 agtaagacca gtagcactaa ttccaagttc cataccccat gaagtgcctc agatattaat
     4681 taatagagaa cctttgcctc atctgcattt tgatgtagag cttcttggag actgtgatgt
     4741 cataattaat gaattgtgtc ataggttagg tggtgaatat gccaaacttt gctgtaaccc
     4801 tgtaaagctt tcagaaatta ctgaaaaacc tccacgaaca caaaaagaat tggcttattt
     4861 gtcagagttg ccacccacac ctcttcatgt ttcagaagac tcaagttcac cagaaagaac
     4921 ttcaccacca gattcttcag tgattgtcac acttttagac caagcagcta agagtaatga
     4981 tgatttagat gtgtctgaat caaaaggttg tatggaagaa aaaccacagg aagtacaaac
     5041 ttctaggaat gttgaaagta ttgctgaaca gatggaaaat ccggatttga agaatgttgg
     5101 ttctagtact ggggagaaaa atgaaagaac ttcagtggct ggaacagtga gaaaatgctg
     5161 gcctaataga gtggcaaagg agcagattag taggcggctt gatggtaatc agtatctgtt
     5221 tttgccacca aatcgttaca ttttccatgg cgctgaggta tattcagact ctgaagatga
     5281 cgtcttatcc tctagttctt gtggcagtaa cagtgatagt gggacatgcc agagtccaag
     5341 tttagaagaa cccatggagg atgaaagtga aattgaagaa ttctacaatg gcttagaaga
     5401 tgagcctgat gttccagaga gagctggagg agctggattt gggactgatg gagatgatca
     5461 agaggcaatt aatgaagcta tatctgtgaa acaggaagta acagacatga actatccatc
     5521 aaacaaatca tagtgtaata attgtgcagg tacaggaatt gttccactct agacaagggc
     5581 gaattcgcgg ccgctaaatt caattcgccc tatagtgagt cgtattacaa ttcactggcc
     5641 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5701 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5761 caacagttgc gcagcctata cgtaggaatt cgccagcaca gtggtcgacc ctgtggaatg
     5821 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5881 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5941 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6001 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6061 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6121 gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg
     6181 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6241 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6301 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6361 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6421 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6481 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6541 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6601 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6661 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6721 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     6781 gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc
     6841 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     6901 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     6961 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     7021 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     7081 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     7141 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     7201 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     7261 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     7321 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     7381 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     7441 gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt
     7501 aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7561 gggcgct
//
