LOCUS       sequence-152141-       12344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12344)
  TITLE     myc mTOR kinase dead
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: myc mTOR kinase dead
FEATURES             Location/Qualifiers
     source          1..12344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        250..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     784..804
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     830..854
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1064..1082
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1064..1081
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1095..1114
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1160..1169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1169..1198
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4053..4062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7274..7546
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     regulatory      8880..8889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8893..9027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8930..8949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8984..9003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9096..9453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9096..9287
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9119..9139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9304..9439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9473..9490)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9473..9489)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9482..9504)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9702..10157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9789..9808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9999..10020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10174..10193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10293..10315
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10353..10371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10439..10543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10544..10612,10613..11404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10762..10781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11575..12163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12064..12083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12317..12334
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg
      241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
     1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
     1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
     1141 cggttctatc gataagcttg ccaccatgga acaaaagctc attagcgagg aagaccttaa
     1201 catgcttggg acaggccctg ccacggccac cgccggtgcc gccacatcta gcaacgtgag
     1261 cgttctgcag cagttcgcca gtgggctgaa gagccggaat gaggagacca gggccaaagc
     1321 agccaaggag ctccagcact atgtcaccat ggaacttcga gagatgagtc aggaggagtc
     1381 tactcgcttc tatgaccagc tgaaccatca catttttgaa ctggtttcca gctcagacgc
     1441 caatgagagg aagggtggca tcttggccat tgccagcctc attggagtgg aaggtgggaa
     1501 ttccaccagg attggcagat ttgccaacta ccttcggaac ctcctcccct caagtgatcc
     1561 agttgtcatg gagatggcat ccaaggccat tggccgcctt gcaatggcag gggacacttt
     1621 cactgctgag tatgtggagt ttgaagtgaa gcgagccttg gagtggctgg gtgctgaccg
     1681 aaatgagggc cggagacatg cagctgtcct cgttctccgt gagctggcca tcagcgtccc
     1741 caccttcttc ttccagcaag ttcagccctt ctttgacaac atttttgtgg ctgtgtggga
     1801 ccccaagcag gccatccgtg aaggagctgt ggctgccctt cgtgcctgtc tgattcttac
     1861 cacgcagcgg gagccgaagg agatgcagaa gcctcagtgg tacaggcaca catttgaaga
     1921 agcagagaaa ggttttgatg agaccctggc caaagagaag ggtatgaacc gagatgatcg
     1981 aatccacggg gccttgctga tcctcaacga gctcgtccga atcagcagca tggagggaga
     2041 gcgtctgaga gaggagatgg aggaaatcac ccagcagcag ctggtacatg acaagtactg
     2101 caaagaccta atgggctttg ggacaaagcc tcggcacatc actcccttca ccagcttcca
     2161 ggctgtgcag ccccagcagt caaacgcctt ggtgggactg ctggggtaca gctcccacca
     2221 aggcctaatg gggtttgggg cttcccccag ccctacaaag tccactctgg tggaaagccg
     2281 ttgttgcaga gacttgatgg aagagaaatt tgatcaggtg tgccagtggg tgctgaaatg
     2341 taggagcagc aagaactcac tgatccaaat gacaatcctt aatctgttgc cccgcttggc
     2401 tgcattccga ccgtctgcct tcacagatac ccagtacctg caagacacca tgaaccatgt
     2461 cctgagctgt gtcaagaagg aaaaggaacg gaccgcagcg ttccaggccc tagggctgct
     2521 ttctgtggcg gtgaggtccg agtttaaggt ctacctgccc cgagtacttg acatcatccg
     2581 agcagccctg cctccaaagg actttgccca caagaggcag aaaactgtgc aggtggatgc
     2641 cacagtgttc acgtgcatca gcatgctggc gcgggccatg gggccaggca tccagcagga
     2701 catcaaggag ctgctggagc ccatgttggc agtgggcctg agccctgcgc tcactgctgt
     2761 gctctatgac ctgagccggc agattccgca gctgaagaaa gatattcagg acgggcttct
     2821 gaagatgctg tccctggtcc ttatgcacaa acccctgcgg cacccgggca tgcccaaagg
     2881 cctggcccac cagctggcct ccccaggtct taccaccctc cctgaggcca gcgacgtggc
     2941 cagcatcact cttgcccttc gaactctcgg cagctttgaa tttgaaggcc actctctgac
     3001 ccagttcgtc cgacactgcg cagatcattt cctgaacagt gagcacaagg agatccgcat
     3061 ggaagcagcc cgcacctgct cccgcctgct cacaccctcc atccacctca tcagcggcca
     3121 tgcccatgtg gttagccaga ccgcagtgca agtggtagca gatgtgctca gcaagctgct
     3181 tgtggtcggc ataacagatc ctgatcctga tatccgctac tgtgtcttgg cctccctgga
     3241 tgagcgcttc gatgcccact tggcccaggc agaaaactta caagctctgt ttgtggctct
     3301 gaatgaccag gtctttgaga tccgagagct ggccatctgc actgtgggcc gactcagtag
     3361 catgaaccca gcctttgtca tgcctttcct gcgcaagatg ctcatccaga ttttgacaga
     3421 gctggagcac agtggcattg ggagaatcaa ggagcagagt gcccgcatgc tggggcacct
     3481 ggtctccaat gccccccgcc tcatccgccc ctatatggag cctattctga aggctttaat
     3541 tttgaaactc aaagatccag accctgaccc aaacccgggc gtgatcaata acgtgttggc
     3601 cactatagga gaactggctc aggttagcgg cctggagatg aggaagtggg tggacgagct
     3661 ctttgtcatc atcatggaca tgctgcagga ctcctctctt ctggccaaaa gacaggtggc
     3721 tttgtggacc ctgggacagt tggtggccag tactggctac gtggtggagc cctacaggaa
     3781 gtaccccact ctgcttgaag tgctgctgaa ttttctgaag acggagcaga accagggcac
     3841 tcggagagag gccatccgag tgttagggct cctcggggct ttggacccct acaagcacaa
     3901 agtgaacatc ggcatgattg accagtcccg agatgcttct gctgtcagcc tgtcagaatc
     3961 caagtcaagt caagattcct ctgactacag caccagtgaa atgctggtca acatgggaaa
     4021 cctgccactg gacgagttct accccgccgt gtccatggtg gccttgatgc ggatcttccg
     4081 agaccagtcc ctctctcacc accacaccat ggtggttcag gccatcacct tcatcttcaa
     4141 gtccctgggg ctcaagtgtg tgcagttcct gccccaggtc atgcccacgt tccttaacgt
     4201 catccgagtc tgtgatgggg ccatccggga atttctgttc cagcagctgg gaatgctggt
     4261 gtcctttgtg aagagccaca tccgtcccta catggatgaa atagtcaccc tcatgagaga
     4321 attttgggtc atgaacacct caatccagag cacaatcatt cttctcattg agcaaatcgt
     4381 ggtggctctt ggaggtgaat ttaagctcta cctgccccag ctgatcccac acatgctgcg
     4441 tgtcttcatg catgacaaca gccagggccg catagtctcc atcaagctgt tagcagcgat
     4501 ccagctgttt ggcgccaacc tggatgacta tctgcacttg ttgttgcctc cgatcgtgaa
     4561 attgtttgat gcccctgaag ttccactgcc gtcgagaaag gcagcgttgg agacagtgga
     4621 ccgcctgaca gagtccctgg atttcactga ctatgcctcc cgcatcattc acccgattgt
     4681 tcgcacgcta gaccagagcc cagagctgcg ctccacagcc atggacaccc tgtcttcact
     4741 tgtgtttcaa ctagggaaga agtaccagat cttcattcca atggtgaata aagtccttgt
     4801 gcgacaccgg atcaatcacc agcgctacga cgtgctgatc tgcagaatcg tcaaggggta
     4861 cacgcttgct gatgaagaag aagacccttt gatttaccag catcgaatgc taaggagcag
     4921 ccagggagat gccctggcca gtggaccagt tgaaacagga cccatgaaga aactgcatgt
     4981 cagcaccatc aacctccaaa aggcctgggg agctgccaga agggtctcca aggacgactg
     5041 gctggagtgg ctgcgacgct tgagtctgga gctgctgaag gattcctcat caccttccct
     5101 gcgctcatgc tgggccctgg cccaggccta caaccccatg gccagggatc tcttcaacgc
     5161 tgcgtttgtg tcctgctggt ctgaactgaa tgaagaccaa caagatgagc tcatcaggag
     5221 cattgagttg gctctcactt ctcaagacat tgctgaagtc acacaaaccc tcttgaactt
     5281 ggctgagttc atggagcaca gtgacaaggg ccccctacca ctgagagatg acaatggcat
     5341 cgtgctgttg ggtgagagag ctgccaagtg ccgggcatat gccaaagcac tacactacaa
     5401 agagctggag ttccagaagg ggcccacgcc tgccatactt gagtccctca tcagcattaa
     5461 taataaactg cagcagcctg aggcagcgtc cggggtgtta gagtacgcca tgaaacactt
     5521 cggagagctg gagatccagg ccacctggta tgagaagttg catgagtggg aggacgccct
     5581 tgtggcctac gacaagaaga tggacacgaa caaggatgac ccagagctga tgctgggccg
     5641 catgcgctgt ctcgaggcct tgggagaatg gggccagctt catcagcagt gctgtgaaaa
     5701 gtggactctg gttaatgacg agacccaggc taagatggcc cggatggctg ctgcagcagc
     5761 atggggttta ggtcagtggg acagcatgga ggagtacacc tgtatgattc ctcgggatac
     5821 tcacgatgga gcattctaca gagcagtgtt ggcactgcat caggatctct tctccttggc
     5881 tcaacagtgc attgacaagg ccagggacct gctggacgcc gagctgactg ccatggcagg
     5941 ggagagctac agccgagcct atggggccat ggtttcttgc cacatgctgt ccgagctgga
     6001 ggaggttatc cagtacaaac tcgtcccgga gcgacgggag atcatccgcc agatctggtg
     6061 ggagagactg cagggctgcc agcgtattgt agaggactgg cagaaaatcc tcatggtccg
     6121 gtcccttgtg gtcagccctc acgaggacat gagaacctgg ctcaagtacg caagcctgtg
     6181 tggcaagagc ggcagactgg ctcttgctca taaaacctta gtgttgctct tgggagttga
     6241 tccatctcgg caacttgacc atcctctgcc aacagttcac cctcaagtga cctatgccta
     6301 catgaaaaac atgtggaaaa gcgctcggaa gattgatgcc ttccagcaca tgcagcactt
     6361 tgtgcagacc atgcagcagc aggcccagca cgccattgcc acagaggacc agcagcacaa
     6421 gcaggagctg cataagctca tggccaggtg ttttctgaaa cttggggagt ggcagctgaa
     6481 cctccagggc atcaacgaga gcaccatccc caaggtgcta cagtactaca gtgctgccac
     6541 agagcatgac cgcagctggt ataaggcttg gcacgcatgg gcagtgatga actttgaagc
     6601 cgtgctacac tacaaacatc agaaccaagc ccgcgatgag aagaagaaac tgcgccatgc
     6661 cagcggggcc aacatcacca atgccaccac cactgccacc accgctgcct ccgctgccgc
     6721 tgccaccagc acagagggca gcaacagtga aagtgaagcc gagagcaatg agagcagccc
     6781 caccccgtcc cctctgcaga agaaggtcac tgaggatttg tccaaaaccc tcttgttgta
     6841 cactgtccct gctgtccaag gcttcttccg ttctatctcc ttgtcgagag gcaacaacct
     6901 ccaggataca ctcagagtcc tcaccttgtg gtttgattat ggtcactggc cagatgtcaa
     6961 tgaagccctg gtggaagggg tgaaggccat acagattgac acttggttac aggttatacc
     7021 tcagctcatt gcaagaattg acacgcccag acccttggtg ggccggctca ttcaccagct
     7081 cctcacagat attggtcggt accacccaca ggccctcatc taccccctga cggtggcttc
     7141 taagtctacc accacagccc gtcacaatgc agccaacaag atcctgaaga acatgtgcga
     7201 gcacagcaac acgctagtcc agcaggccat gatggtgagt gaagagctga ttcgagtagc
     7261 catcctctgg catgagatgt ggcatgaagg cctagaagag gcctctcgct tgtactttgg
     7321 ggagaggaac gtcaaaggca tgtttgaggt gctggagccc ctgcatgcta tgatggaacg
     7381 cggtccccag accctgaagg aaacgtcctt taatcaggca tatggtcgag atttaatgga
     7441 ggcacaagaa tggtgccgaa agtacatgaa atcagggaac gtcaaggacc tcacccaagc
     7501 ctgggacctc tactatcacg tgttcagacg gatctccaag cagctaccac agctcacatc
     7561 cctggagctg cagtatgtgt cccccaaact tttgatgtgc agagaccttg aattggctgt
     7621 gccaggaaca tatgacccca accagccaat cattcgcatt cagtccatag ccccgtcttt
     7681 gcaagtcatc acatccaagc agaggcctcg gaagctgacc ctgatgggca gcaatgggca
     7741 cgagtttgtt ttcctcctga aaggccatga agatctgcgg caggacgagc gagtgatgca
     7801 gctctttggc ctggtgaaca cactcctagc caatgaccca acttctcttc gaaagaacct
     7861 cagcatccag agatacgccg tcattcctct gtccaccaac tcgggcctga ttggctgggt
     7921 gccccactgt gacacgctgc atgccctcat ccgggactac agagagaaga agaagatcct
     7981 gctgaacatc gagcaccgca tcatgctgcg gatggctcct gactatgacc acctgactct
     8041 gatgcagaag gtggaggtgt ttgagcatgc tgtcaacaac acagccgggg atgacctggc
     8101 caagctgctg tggctgaaaa gccccagctc agaggtgtgg tttgaccgaa gaaccaatta
     8161 tactcgctcc ctggctgtca tgtccatggt tggatacatt ttaggccttg gagacaggca
     8221 cccatccaac ctgatgctgg accggctgag tggaaagatc ctgcacattg agtttgggga
     8281 ctgctttgag attgctatga ccagagagaa atttccagaa aagattccat ttagactaac
     8341 aagaatgttg accaatgcta tggaggttac cggtctcgat ggcaactata gaaccacatg
     8401 ccacacagtg atggaggtgc ttcgggagca caaggacagt gtcatggctg tgctagaagc
     8461 ctttgtctat gaccctctgc tgaattggag gctgatggac acaaatgcca aaggcaacaa
     8521 gcggtcccga accaggacag actcctattc tgcaggccag tcagtagaaa ttttggacgg
     8581 tgtagaactt ggagaaccag cccataagaa aacagggacc actgtgccag aatccatcca
     8641 ttctttcatt ggagatggtt tggtgaaacc agaagcctta aacaagaaag ctattcagat
     8701 tattaacagg gttcgagata agctcactgg tcgggatttc tctcatgatg acactttgga
     8761 tgttccaacc caagtggaac tgcttatcaa gcaagcgaca tctcatgaga acctctgcca
     8821 gtgctacatt ggctggtgtc ctttctggta atagtctaga gtcgacctgc agaagcttgg
     8881 ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9001 atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc atggcctgaa
     9061 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
     9121 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9181 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9241 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9361 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9421 ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg gcactggccg
     9481 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9541 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9601 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     9661 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     9721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9901 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10021 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
    10081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    10141 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    10261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    10321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
    10381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
    10441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    10801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    11401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    11461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    11581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    11641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    11701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    11761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    11821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    12181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    12241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    12301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
