LOCUS       sequence-152142-        6270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Crane R, Kloepfer A, Ruderman JV
  TITLE     Requirements for the destruction of human Aurora-A.
  JOURNAL   J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9.
  PUBMED    15536123
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci"; date: "2004-12-1"; volume: "117(Pt 25)"; pages: "5975-83. Epub
            2004 Nov 9"
COMMENT     Sequence Label: hAur-A D274A
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     497..519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     535..551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1481..1492
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1809..1829)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1814..2143
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1994..2129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2153..2752
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2153..2172)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2649..2669
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2973..3442
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3572..3589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3743..4331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3823..3842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4491..5282,5283..5351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5114..5133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5352..5456)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5487..5956
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6021..6220
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
       61 cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg
      121 actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg
      181 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
      241 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
      301 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
      361 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
      421 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
      481 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
      541 ccagccctca ctccttctct aggcgccggc cggatccacc atggaccgat ctaaagaaaa
      601 ctgcatttca ggacctgtta aggctacagc tccagttgga ggtccaaaac gtgttctcgt
      661 gactcagcaa tttccttgtc agaatccatt acctgtaaat agtggccagg ctcagcgggt
      721 cttgtgtcct tcaaattctt cccagcgcgt tcctttgcaa gcacaaaagc ttgtctccag
      781 tcacaagccg gttcagaatc agaagcagaa gcaattgcag gcaaccagtg tacctcatcc
      841 tgtctccagg ccactgaata acacccaaaa gagcaagcag cccctgccat cggcacctga
      901 aaataatcct gaggaggaac tggcatcaaa acagaaaaat gaagaatcaa aaaagaggca
      961 gtgggctttg gaagactttg aaattggtcg ccctctgggt aaaggaaagt ttggtaatgt
     1021 ttatttggca agagaaaagc aaagcaagtt tattctggct cttaaagtgt tatttaaagc
     1081 tcagctggag aaagccggag tggagcatca gctcagaaga gaagtagaaa tacagtccca
     1141 ccttcggcat cctaatattc ttagactgta tggttatttc catgatgcta ccagagtcta
     1201 cctaattctg gaatatgcac cacttggaac agtttataga gaacttcaga aactttcaaa
     1261 gtttgatgag cagagaactg ctacttatat aacagaattg gcaaatgccc tgtcttactg
     1321 tcattcgaag agagttattc atagagacat taagccagag aacttacttc ttggatcagc
     1381 tggagagctt aaaattgcag cttttgggtg gtcagtacat gctccatctt ccaggaggac
     1441 cactctctgt ggcaccctgg actacctgcc ccctgaaatg attgaaggtc ggatgcatga
     1501 tgagaaggtg gatctctgga gccttggagt tctttgctat gaatttttag ttgggaagcc
     1561 tccttttgag gcaaacacat accaagagac ctacaaaaga atatcacggg ttgaattcac
     1621 attccctgac tttgtaacag agggagccag ggacctcatt tcaagactgt tgaagcataa
     1681 tcccagccag aggccaatgc tcagagaagt acttgaacac ccctggatca cagcaaattc
     1741 atcaaaaccg tcaaattgcc aaaacaaaga atcagctagc aaacagtctt aggaatcctc
     1801 gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     1861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     1921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     1981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga
     2161 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     2221 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     2281 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     2341 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     2401 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     2461 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     2521 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     2581 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     2641 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     2701 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     2761 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga taaaataaaa
     2821 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     2881 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     2941 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3001 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     3061 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     3121 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     3181 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     3241 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     3301 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     3361 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     3421 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct
     3481 taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag
     3541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4441 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4501 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4561 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4621 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4681 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4741 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4801 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4861 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4921 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4981 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5041 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5101 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5161 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5221 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5281 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5341 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5401 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5461 tttccccgaa aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag
     5521 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     5581 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     5641 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     5701 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     5761 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     5821 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     5881 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     5941 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
     6001 ccgacccacc accgggaggc aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
     6061 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
     6121 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt
     6181 cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat
     6241 ccgaccccgt caggatatgt ggttctggta
//
