LOCUS       sequence-152152-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYOD1-3xHA (#730)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(185..211)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    264..288
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(311..391)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgtaggaggg
       61 gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca gatcttgggt gggttactcc
      121 agactgcctt gggaaaagcg cctcccctac ccggtagaat ttctagttta attaatcatt
      181 actaagcgta gtctgggacg tcgtatgggt attcgaaccg cgggccctct agactcgagc
      241 ggccgccact gtgctggata tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac
      301 ccttctatta agcgtagtca ggtacatcgt aagggtaagc gtaatccgga acgtcgtacg
      361 gatacgcgta gtctggaacg tcatatgggt actcgaggag cacctggtat atcgggttgg
      421 ggttcgcacc cgcagggcac tgcggggcgg cgtccggtga ctgggtgggg tcgccgctgc
      481 tctctccctc gctgggggcg gcagcctctt gcctgcgcgg aggcgactca gaaggcacgt
      541 ccgccagcag gagggcgggc gccgcagggc tctcggtgga gatgcgctcc acgatgctgg
      601 acaggcagtc taggctcgac accgccgcac tcttcccggg cctgggttcg ctgggcgcct
      661 cgttgtagta ggcgccttcg tagcagttcc gccgccgggc gccgctcggg gggccgctgt
      721 agtccatcat gccgtcggag cagttggagc gcgggctgga cgcgtcggag tcgccgctgt
      781 agtgctcgcc gccgcggccc gggggcagcg ggcccggcgc atagaaggcg gctgcggcgc
      841 caggg
//
