LOCUS       sequence-152164-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human MYOD1 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hMYOD1 (#794)
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1060..1076)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1061..1077)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
       61 gagcccgggc ggatccatgg agctactgtc gccaccgctc cgcgacgtag acctgacggc
      121 ccccgacggc tctctctgct cctttgccac aacggacgac ttctatgacg acccgtgttt
      181 cgactccccg gacctgcgct tcttcgaaga cctggacccg cgcctgatgc acgtgggcgc
      241 gctcctgaaa cccgaagagc actcgcactt ccccgcggcg gtgcacccgg ccccgggcgc
      301 acgtgaggac gagcatgtgc gcgcgcccag cgggcaccac caggcgggcc gctgcctact
      361 gtgggcctgc aaggcgtgca agcgcaagac caccaacgcc gaccgccgca aggccgccac
      421 catgcgcgag cggcgccgcc tgagcaaagt aaatgaggcc tttgagacac tcaagcgctg
      481 cacgtcgagc aatccaaacc agcggttgcc caaggtggag atcctgcgca acgccatccg
      541 ctatatcgag ggcctgcagg ctctgctgcg cgaccaggac gccgcgcccc ctggcgccgc
      601 agccgccttc tatgcgccgg gcccgctgcc cccgggccgc ggcggcgagc actacagcgg
      661 cgactccgac gcgtccagcc cgcgctccaa ctgctccgac ggcatgatgg actacagcgg
      721 ccccccgagc ggcgcccggc ggcggaactg ctacgaaggc gcctactaca acgaggcgcc
      781 cagcgaaccc aggcccggga agagtgcggc ggtgtcgagc ctagactgcc tgtccagcat
      841 cgtggagcgc atctccaccg agagccctgc ggcgcccgcc ctcctgctgg cggacgtgcc
      901 ttctgagtcg cctccgcgca ggcaagaggc tgccgccccc agcgagggag agagcagcgg
      961 cgaccccacc cagtcaccgg acgccgcccc gcagtgccct gcgggtgcga accccaaccc
     1021 gatataccag gtgctctgag aattcgatat caagcttatc gataccgtcg acctcgaggg
     1081 ggacagtacc tatataggta cagtaggtac caattcgcct aatacagt
//
