LOCUS       sequence-152167-        6416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6416)
  TITLE     Other Melton Lab plasmids
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Hsp68-CreER(TM) (DM#264)
FEATURES             Location/Qualifiers
     source          1..6416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     301..317
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     302..318
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1231..2259
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    3640..3774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3677..3696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3731..3750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3846..3865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3847..3865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3872..3889)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3872..3888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3881..3903)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4030..4485
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4117..4136)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4327..4348
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4511..4615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4616..4684,4685..5476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4834..4853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5647..6235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6136..6155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6389..6406
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tgggtacggc cggccgtacc gggccccccc
      301 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagccccgc gggtaccgag
      361 ctccaggaac atccaaactg agcagccggg gtccccccca ccccccaccc cgcccctccc
      421 ggcaactttg agcctgtgct gggacagagc ctctagttcc taaattagtc catgaggtca
      481 gaggcagcac tgccattgta acgcgattgg agaggatcac gtcaccggac acgcccccag
      541 gcatctccct gggtctccta aacttggcgg ggagaagttt tagcccttaa gttttagcct
      601 ttaaccccca tattcagaac tgtgcgagtt ggcgaaaccc cacaaatcac aacaaactgt
      661 acacaacacc gagctagagg tgatctttct tgtccattcc acacaggcct tagtaatgcg
      721 tcgccatagc aacagtgtca ctagtagcac cagcacttcc ccacaccctc cccctcagga
      781 atccgtactc tccagtgaac cccagaaacc tctggagagt tctggacaag ggcggaaccc
      841 acaactccga ttactcaagg gaggcgggga agctccacca gacgcgaaac tgctggaaga
      901 ttcctggccc caaggcctcc tccggctcgc tgattggccc agcggagagt gggcggggcc
      961 ggtgaagact ccttaaaggc gcagggcggc gagcaggtca ccagacgctg acagctactc
     1021 agaaccaaat ctggttccat ccagagacaa gcgaagacaa gagaagcaga gcgagcggcg
     1081 cgttcccgat cctcggccag gaccagcctt ccccagagca tccctgccgc ggagcgcaac
     1141 cttcccagga gcatccctgc cgcggagcgc aactttcccc ggagcatcca cgccgcggag
     1201 cgcagccttc cagaagcaga gcgcggcgcc atgtccaatt tactgaccgt acaccaaaat
     1261 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg
     1321 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg
     1381 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat
     1441 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa
     1501 catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc
     1561 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt
     1621 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa
     1681 aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc
     1741 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt
     1801 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag
     1861 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta
     1921 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca
     1981 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga
     2041 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt
     2101 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg
     2161 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt
     2221 gaaacagggg caatggtgcg cctgctggaa gatggggatc aggctggtgc catgggcgat
     2281 ccacgaaatg aaatgggtgc ttcaggagac atgagggctg ccaacctttg gccaagccct
     2341 cttgtgatta agcacactaa gaagaatagc cctgccttgt ccttgacagc tgaccagatg
     2401 gtcagtgcct tgttggatgc tgaaccgccc atgatctatt ctgaatatga tccttctaga
     2461 cccttcagtg aagcctcaat gatgggctta ttgaccaacc tagcagatag ggagctggtt
     2521 catatgatca actgggcaaa gagagtgcca ggctttgggg acttgaatct ccatgatcag
     2581 gtccaccttc tcgagtgtgc ctggctggag attctgatga ttggtctcgt ctggcgctcc
     2641 atggaacacc cggggaagct cctgtttgct cctaacttgc tcctggacag gaatcaaggt
     2701 aaatgtgtgg aaggcatggt ggagatcttt gacatgttgc ttgctacgtc aagtcggttc
     2761 cgcatgatga acctgcaggg tgaagagttt gtgtgcctca aatccatcat tttgcttaat
     2821 tccggagtgt acacgtttct gtccagcacc ttgaagtctc tggaagagaa ggaccacatc
     2881 caccgtgtcc tggacaagat cacagacact ttgatccacc tgatggccaa agctggcctg
     2941 actctgcagc agcagcatcg ccgcctagct cagctccttc tcattctttc ccatatccgg
     3001 catatgagta acaaacgcat ggagcatctc tacaacatga aatgcaagaa cgtggtaccc
     3061 ctctatgacc tgctcctgga gatgttggat gcccaccgcc ttcatgcccc agccagtcgc
     3121 atgggagtgc ccccagagga gcccagccag acccagctgg ccaccaccag ctccacttca
     3181 gcacattcct tacaaaccta ctacataccc ccggaagcag agggcttccc caacacgatc
     3241 tgagagctcc tactagtcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg
     3301 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     3361 agtgtataat gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag
     3421 tatgatttat gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag
     3481 attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata
     3541 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3601 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatctg
     3781 cggccgccac cgcggtggag ctgtttaaac agctgtttaa acagctgttt aaacgctcca
     3841 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     3901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     3961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4021 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4441 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
