LOCUS       sequence-152168-        7213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Smogorzewska A, de Lange T
  TITLE     Different telomere damage signaling pathways in human and mouse 
            cells.
  JOURNAL   EMBO J. 2002 Aug 15. 21(16):4338-48.
  PUBMED    12169636
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2002 Aug 15. 21(16):4338-48."
COMMENT     Sequence Label: pLPC-NMYC TRF2
FEATURES             Location/Qualifiers
     source          1..7213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        159..538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     693..713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     787..806
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        787..805
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             846..875
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     LTR             2611..3080
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3210..3227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3381..3969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3461..3480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4129..4920,4921..4989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4752..4771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4990..5094)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5125..5594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5659..5858
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6059..6475
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6405..6427
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6443..6459
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             6493..7092
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6493..6512)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6989..7009
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7181..7200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
       61 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      121 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      721 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      781 cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc ggatcagcca
      841 ccatggaaca aaagttgatt tctgaagaag atttgttaag aaagagagga tccgcgggag
      901 gaggcgggag tagcgacggc agcgggcggg cagctggcag gcgggcgtcc cgcagtagcg
      961 ggcgggcccg gcgggggcgc cacgagccgg ggctgggggg cccggcggag cgcggcgcgg
     1021 gggaggcacg gctggaagag gcagtcaatc gctgggtgct caagttctac ttccacgagg
     1081 cgctgcgggc ctttcggggt agccggtacg gggacttcag acagatccgg gacatcatgc
     1141 aggctttgct tgtcaggccc ttggggaagg agcacaccgt gtcccgattg ctgcgggtta
     1201 tgcagtgtct gtcgcggatt gaagaagggg aaaatttaga ctgttccttt gatatggagg
     1261 ctgagctcac accactggaa tcagctatca atgtgctgga gatgattaaa acggaattta
     1321 cactgacaga agcagtggtc gaatccagta gaaaactggt caaggaagct gctgtcatta
     1381 tttgtatcaa aaacaaagaa tttgaaaagg cttcaaaaat tttgaaaaaa catatgtcca
     1441 aggaccccac aactcagaag ctgagaaatg atctcctgaa tattattcga gaaaagaact
     1501 tggcccatcc tgttatccag aacttttcat atgagacctt ccagcagaag atgctgcgct
     1561 tcctggagag ccacctggat gacgccgagc cctacctcct cacgatggcc aaaaaggctt
     1621 tgaaatctga gtccgctgcc tcaagtacag ggaaggaaga taaacagcca gcaccagggc
     1681 ctgtggaaaa gccacccaga gaacccgcaa ggcagctacg gaatcctcca accaccattg
     1741 gaatgatgac tctgaaagca gctttcaaga ctctgtctgg tgcacaggat tctgaggcag
     1801 cctttgcaaa actggaccag aaggatctgg ttcttcctac tcaagctctc ccagcatcac
     1861 cagccctcaa aaacaagaga cccagaaaag atgaaaacga aagttcagcc ccggctgacg
     1921 gtgagggtgg ctcggaactg cagcccaaga acaagcgcat gacaataagc agattggtct
     1981 tggaggagga cagccagagt actgagccca gcgcaggcct caactcctcc caggaggccg
     2041 cttcagcgcc accatccaag cccaccgttc tcaaccaacc cctccctgga gagaagaatc
     2101 ccaaagtacc caaaggcaag tggaacagct ctaatggggt tgaagaaaag gagacttggg
     2161 tggaagagga tgaactgttt caagttcagg caccagatga agacagtaca accaatataa
     2221 caaaaaagca gaagtggact gtagaagaaa gcgagtgggt caaggctgga gtgcagaaat
     2281 atggggaagg aaactgggct gccatttcta aaaattaccc atttgttaac cgaacagctg
     2341 tgatgattaa ggatcgctgg cggaccatga aaagacttgg catgaactga gaattctgca
     2401 gatatccatc acactggcgg ccgctcgagc atgcatctag agggccgata aaataaaaga
     2461 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     2521 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     2581 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     2641 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
     2701 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     2761 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     2821 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     2881 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
     2941 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     3001 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     3061 ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta
     3121 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc
     3181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
     4081 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4141 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4201 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4261 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4321 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4381 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4441 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4501 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4561 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4621 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4681 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4741 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4801 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4861 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4921 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4981 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5041 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5101 tccccgaaaa gtgccacctg cagcctgaat atgggccaaa caggatatct gtggtaagca
     5161 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     5221 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     5281 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     5341 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     5401 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     5461 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     5521 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     5581 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
     5641 gacccaccac cgggaggcaa gctggccagc aacttatctg tgtctgtccg attgtctagt
     5701 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
     5761 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
     5821 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
     5881 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
     5941 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     6001 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     6061 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     6121 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     6181 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     6241 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     6301 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     6361 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     6421 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggccg
     6481 gatcagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6541 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6601 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6661 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6721 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6781 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6841 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6901 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6961 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     7021 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     7081 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     7141 catgcatcgg atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     7201 catagttaag cca
//
