LOCUS       sequence-152200-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYF5-3xHA (#732)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
      121 accgccacca tggacatgac ggacggctgc cagttctcac cttctgagta cttctacgac
      181 ggctcctgca taccgtcccc cgagggtgaa tttggggacg agtttgtgcc gcgagtggct
      241 gccttcggag cgcacaaagc agagctgcag ggctcagatg aggacgagca cgtgcgagcg
      301 cctaccggcc accaccaggc tggtcactgc ctcatgtggg cctgcaaagc ctgcaagagg
      361 aagtccacca ccatggatcg gcggaaggca gccactatgc gcgagcggag gcgcctgaag
      421 aaggtcaacc aggctttcga aaccctcaag aggtgtacca cgaccaaccc caaccagagg
      481 ctgcccaagg tggagatcct caggaatgcc atccgctaca tcgagagcct gcaggagttg
      541 ctgagagagc aggtggagaa ctactatagc ctgccgggac agagctgctc ggagcccacc
      601 agccccacct ccaactgctc tgatggcatg cccgaatgta acagtcctgt ctggtccaga
      661 aagagcagta cttttgacag catctactgt cctgatgtat caaatgtata tgccacagat
      721 aaaaactcct tatccagctt ggattgctta tccaacatag tggaccggat cacctcctca
      781 gagcaacctg ggttgcctct ccaggatctg gcttctctct ctccagttgc cagcaccgat
      841 tcacagcctg caactccagg
//
