LOCUS       sequence-152202-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYF5-3xHA (#732)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(184..210)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    263..287
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(310..390)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
       61 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg ggttactcca
      121 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tctagtttaa ttaatcatta
      181 ctaagcgtag tctgggacgt cgtatgggta ttcgaaccgc gggccctcta gactcgagcg
      241 gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
      301 cttctattaa gcgtagtcag gtacatcgta agggtaagcg taatccggaa cgtcgtacgg
      361 atacgcgtag tctggaacgt catatgggta ctcgagtcga cgcatgcgaa ttcgtagcac
      421 atgatagata agcctggaac tagaagcccc tggagttgca ggctgtgaat cggtgctggc
      481 aactggagag agagaagcca gatcctggag aggcaaccca ggttgctctg aggaggtgat
      541 ccggtccact atgttggata agcaatccaa gctggataag gagtttttat ctgtggcata
      601 tacatttgat acatcaggac agtagatgct gtcaaaagta ctgctctttc tggaccagac
      661 aggactgtta cattcgggca tgccatcaga gcagttggag gtggggctgg tgggctccga
      721 gcagctctgt cccggcaggc tatagtagtt ctccacctgc tctctcagca actcctgcag
      781 gctctcgatg tagcggatgg cattcctgag gatctccacc ttgggcagcc tctggttggg
      841 gttggtcgtg gtacacctct tgagggtt
//
