LOCUS       sequence-152203-        7019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM
  TITLE     Suppression of TNF-alpha-induced apoptosis by NF-kappaB.
  JOURNAL   Science. 1996 Nov 1. 274(5288):787-9.
  PUBMED    8864120
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1996 Nov 1. 274(5288):787-9."
COMMENT     Sequence Label: pLXSN IkB alpha M
FEATURES             Location/Qualifiers
     source          1..7019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             588..763
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    826..1025
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1226..1642
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1572..1594
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1610..1626
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2817..2837)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2822..3151
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3002..3137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3211..4005
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3265..3284)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3875..3894
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4065..4658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4761..4783)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4869..5009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4924..4943
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5024..5041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5195..5783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5275..5294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5954..6745,6746..6814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6577..6596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6815..6919)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6987..7005
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
      181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
      241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
      301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
      361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
      421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
      481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
      661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
      721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
      781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1621 cctcactcct tctctaggcg ccggaattcg ttatcaggat cccccgggct gcaggaattc
     1681 ccagcagcag cagcaacaac agtcagacct cgccagccag ccgccagcag cctgcagcct
     1741 gcacccgctc agccccgcac agccatgttt cagccagctg ggcacggcca ggactgggcc
     1801 atggagggcc cgcgggatgg cctcaagaag gagcgcttgg tggacgatcg ccacgacgca
     1861 ggtctagacg ccatgaagga cgaggagtac gagcaaatgg tgaaggagct gcgggagatc
     1921 cgcctgcagc cgcaggaggc gccgctggcc gccgagccct ggaagcagca gctcacggag
     1981 gacggagact cgttcctgca cttggcaatc atccacgaag agaagccgct gaccatggaa
     2041 gtcattggtc aggtgaaggg agacctggcc ttcctcaact tccagaacaa cctgcagcag
     2101 actccactcc acttggctgt gatcaccaac cagccaggaa ttgctgaggc acttctgaaa
     2161 gctggctgtg atcctgagct ccgagacttt cgaggaaata cccctctaca tcttgcctgt
     2221 gagcagggct gcctggccag tgtagcagtc ttgacgcaga cctgcacacc ccagcatctc
     2281 cactccgtcc tgcaggccac caactacaat ggccacacgt gtctgcacct agcctctatc
     2341 cacggctacc tggccatcgt ggagcacttg gtgactttgg gtgctgatgt caacgctcag
     2401 gagccctgca atggccggac agccctccac cttgcggtgg acctgcagaa tcctgacctg
     2461 gtttcgctct tgttgaaatg tggggctgat gtcaacaggg taacctacca aggctactcc
     2521 ccctaccagc ttacctgggg ccgcccaagt acccggatac agcagcagct gggccagctg
     2581 accttggaaa atctccagat gctacctgag gccgaggatg aggaggccta tgacgcggag
     2641 gcagagttcg cagaggatga gctgcccgat gatgactgtg tgtttggagg ccagcgtctg
     2701 acattataag tggaaagtgg caaaaagaat gtggacttgt atatttgtac aaatagagtt
     2761 ttatttttct aaaaaaaaaa aaaaaaagga attcgataac tcgaggatcc ggctgtggaa
     2821 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2881 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2941 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3001 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3061 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3121 aggctttttt ggaggcctag gcttttgcaa aaagcttggg ctgcaggtcg aggcggatct
     3181 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3241 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3301 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     3361 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     3421 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     3481 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     3541 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     3601 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     3661 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     3721 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     3781 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     3841 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     3901 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     3961 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     4021 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     4081 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     4141 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     4201 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     4261 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4321 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     4381 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     4441 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     4501 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     4561 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     4621 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     4681 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt
     4741 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4801 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4861 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     4921 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4981 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     5041 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5761 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5881 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5941 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6001 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6061 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6121 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6181 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6241 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     6301 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6361 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6421 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6481 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6541 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     6601 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6661 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6721 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6781 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6841 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6901 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6961 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
//
