LOCUS       sequence-152213-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Experimental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO-mCherry MALAT1_Exon.2
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..107
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..830
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(265..283)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     533..552
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(899..919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg ggagagggca
       61 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca
      121 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
      181 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      241 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
      301 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
      361 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      421 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      481 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      541 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      601 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
      661 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
      721 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
      781 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa acgcgttaag
      841 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      901 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      961 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct cttt
//
