LOCUS       sequence-152218-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human MYF5 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hMYF5 (#795)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatgg acgtgatgga tggctgccag ttctcacctt ctgagtactt
      181 ctacgacggc tcctgcatac cgtcccccga gggtgaattt ggggacgagt ttgtgccgcg
      241 agtggctgcc ttcggagcgc acaaagcaga gctgcagggc tcagatgagg acgagcacgt
      301 gcgagcgcct accggccacc accaggctgg tcactgcctc atgtgggcct gcaaagcctg
      361 caagaggaag tccaccacca tggatcggcg gaaggcagcc actatgcgcg agcggaggcg
      421 cctgaagaag gtcaaccagg ctttcgaaac cctcaagagg tgtaccacga ccaaccccaa
      481 ccagaggctg cccaaggtgg agatcctcag gaatgccatc cgctacatcg agagcctgca
      541 ggagttgctg agagagcagg tggagaacta ctatagcctg ccgggacaga gctgctcgga
      601 gcccaccagc cccacctcca actgctctga tggcatgccc gaatgtaaca gtcctgtctg
      661 gtccagaaag agcagtactt ttgacagcat ctactgtcct gatgtatcaa atgtatatgc
      721 cacagataaa aactccttat ccagcttgga ttgcttatcc aacatagtgg accggatcac
      781 ctcctcagag caacctgggt tgcctctcca ggatctggct tctctctctc cagttgccag
      841 caccgattca cagcctgcaa ctccagg
//
