LOCUS       sequence-152220-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human MYF5 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hMYF5 (#795)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..34
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     19..35
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(839..862)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      862..871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattctcata
       61 gcacatgata gataagcctg gaactagaag cccctggagt tgcaggctgt gaatcggtgc
      121 tggcaactgg agagagagaa gccagatcct ggagaggcaa cccaggttgc tctgaggagg
      181 tgatccggtc cactatgttg gataagcaat ccaagctgga taaggagttt ttatctgtgg
      241 catatacatt tgatacatca ggacagtaga tgctgtcaaa agtactgctc tttctggacc
      301 agacaggact gttacattcg ggcatgccat cagagcagtt ggaggtgggg ctggtgggct
      361 ccgagcagct ctgtcccggc aggctatagt agttctccac ctgctctctc agcaactcct
      421 gcaggctctc gatgtagcgg atggcattcc tgaggatctc caccttgggc agcctctggt
      481 tggggttggt cgtggtacac ctcttgaggg tttcgaaagc ctggttgacc ttcttcaggc
      541 gcctccgctc gcgcatagtg gctgccttcc gccgatccat ggtggtggac ttcctcttgc
      601 aggctttgca ggcccacatg aggcagtgac cagcctggtg gtggccggta ggcgctcgca
      661 cgtgctcgtc ctcatctgag ccctgcagct ctgctttgtg cgctccgaag gcagccactc
      721 gcggcacaaa ctcgtcccca aattcaccct cgggggacgg tatgcaggag ccgtcgtaga
      781 agtactcaga aggtgagaac tggcagccat ccatcacgtc catggatccg cccgggctct
      841 tatcgtcgtc atccttgtaa tccatggtgg cggccgcca
//
