LOCUS       sequence-152234-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYF5 (#801)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg
       61 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct
      121 tcaccgccac catggattac aaggatgacg acgataagag cccgggcgga tccatggacg
      181 tgatggatgg ctgccagttc tcaccttctg agtacttcta cgacggctcc tgcataccgt
      241 cccccgaggg tgaatttggg gacgagtttg tgccgcgagt ggctgccttc ggagcgcaca
      301 aagcagagct gcagggctca gatgaggacg agcacgtgcg agcgcctacc ggccaccacc
      361 aggctggtca ctgcctcatg tgggcctgca aagcctgcaa gaggaagtcc accaccatgg
      421 atcggcggaa ggcagccact atgcgcgagc ggaggcgcct gaagaaggtc aaccaggctt
      481 tcgaaaccct caagaggtgt accacgacca accccaacca gaggctgccc aaggtggaga
      541 tcctcaggaa tgccatccgc tacatcgaga gcctgcagga gttgctgaga gagcaggtgg
      601 agaactacta tagcctgccg ggacagagct gctcggagcc caccagcccc acctccaact
      661 gctctgatgg catgcccgaa tgtaacagtc ctgtctggtc cagaaagagc agtacttttg
      721 acagcatcta ctgtcctgat gtatcaaatg tatatgccac agataaaaac tccttatcca
      781 gcttggattg cttatccaac atagtggacc ggatcacctc ctcagagcaa cctgggttgc
      841 ctctccagga tctggcttct ctctctccag ttgccagcac cgattcacag cctgcaactc
      901 cagg
//
