LOCUS       sequence-152236-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYF5 (#801)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(184..210)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    263..287
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
       61 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg ggttactcca
      121 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tctagtttaa ttaatcatta
      181 ctaagcgtag tctgggacgt cgtatgggta ttcgaaccgc gggccctcta gactcgagcg
      241 gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
      301 ctttcatagc acatgataga taagcctgga actagaagcc cctggagttg caggctgtga
      361 atcggtgctg gcaactggag agagagaagc cagatcctgg agaggcaacc caggttgctc
      421 tgaggaggtg atccggtcca ctatgttgga taagcaatcc aagctggata aggagttttt
      481 atctgtggca tatacatttg atacatcagg acagtagatg ctgtcaaaag tactgctctt
      541 tctggaccag acaggactgt tacattcggg catgccatca gagcagttgg aggtggggct
      601 ggtgggctcc gagcagctct gtcccggcag gctatagtag ttctccacct gctctctcag
      661 caactcctgc aggctctcga tgtagcggat ggcattcctg aggatctcca ccttgggcag
      721 cctctggttg gggttggtcg tggtacacct cttgagggtt tcgaaagcct ggttgacctt
      781 cttcaggcgc ctccgctcgc gcatagtggc tgccttccgc cgatccatgg tggtggactt
      841 cctcttgcag gctt
//
