LOCUS       sequence-152239-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYF5 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hMYF5 (#801)
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(924..948)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1001..1027
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcggtggccc gaactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
       61 aaaagcaggc tccgcggccg cccccttcac cgccaccatg gattacaagg atgacgacga
      121 taagagcccg ggcggatcca tggacgtgat ggatggctgc cagttctcac cttctgagta
      181 cttctacgac ggctcctgca taccgtcccc cgagggtgaa tttggggacg agtttgtgcc
      241 gcgagtggct gccttcggag cgcacaaagc agagctgcag ggctcagatg aggacgagca
      301 cgtgcgagcg cctaccggcc accaccaggc tggtcactgc ctcatgtggg cctgcaaagc
      361 ctgcaagagg aagtccacca ccatggatcg gcggaaggca gccactatgc gcgagcggag
      421 gcgcctgaag aaggtcaacc aggctttcga aaccctcaag aggtgtacca cgaccaaccc
      481 caaccagagg ctgcccaagg tggagatcct caggaatgcc atccgctaca tcgagagcct
      541 gcaggagttg ctgagagagc aggtggagaa ctactatagc ctgccgggac agagctgctc
      601 ggagcccacc agccccacct ccaactgctc tgatggcatg cccgaatgta acagtcctgt
      661 ctggtccaga aagagcagta cttttgacag catctactgt cctgatgtat caaatgtata
      721 tgccacagat aaaaactcct tatccagctt ggattgctta tccaacatag tggaccggat
      781 cacctcctca gagcaacctg ggttgcctct ccaggatctg gcttctctct ctccagttgc
      841 cagcaccgat tcacagcctg caactccagg ggcttctagt tccaggctta tctatcatgt
      901 gctatgaaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatccagca
      961 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa tacccatacg acgtcccaga
     1021 ctacgcttag taatgattaa ttaaactaga aattctaccg ggtagggta
//
