LOCUS       sequence-152243-       15473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of human POLQ double mutant (in POL and HDL 
            domains).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, 
            Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
  TITLE     Mechanism of suppression of chromosomal instability by DNA 
            polymerase POLQ.
  JOURNAL   PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 
            10.1371/journal.pgen.1004654. eCollection 2014 Oct.
  PUBMED    25275444
REFERENCE   2  (bases 1 to 15473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-10-2"; volume: "10(10):e1004654. doi"; pages: "
            10.1371/journal.pgen.1004654. eCollection 2014 Oct"
COMMENT     Sequence Label: pCDH-EF1-FHC-POLQ-DM
FEATURES             Location/Qualifiers
     source          1..15473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1494..1538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1805..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1964..2175
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2188..2456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             10305..10328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10344..10370
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             10344..10370
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    10381..10953
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10548..10565)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10775..10794
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10962..11561
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10962..10981)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11458..11478
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11568..12156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11621..11641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12039..12050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12230..12463
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12535..12656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12572..12591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12626..12645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12675..12810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12848..12864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12848..12864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12861..12883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12872..12888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12896..12902,12903..12920,12921..12926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12941..12962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13079..13096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13250..13838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14009..14800,14801..14869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14632..14651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14870..14974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15042..15060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15098..15120)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     15220..15239
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15433..15455
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15447..15464
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15448..15464
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     1801 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     1861 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
     1921 tatcgatact agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     1981 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     2041 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     2101 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     2161 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     2221 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     2281 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     2341 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     2401 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     2461 ctgtgaccgg cgcctactct agagctagcg aattcgaatt taaatcggat cctagcacca
     2521 tgaatcttct gcgtcggagt gggaaacggc ggcgttcaga atcaggctca gattcgttct
     2581 cgggaagcgg cggtgacagc agtgccagcc cccagttcct ctccgggtcc gtgctgagcc
     2641 cgccgcccgg ccttggtcgc tgcctgaagg ccgcagctgc aggagaatgc aagcctacag
     2701 ttcctgacta cgaaatagac aagctactat tggcaaactg gggacttcct aaagcagttc
     2761 tggaaaaata ccacagtttt ggtgtaaaaa agatgtttga atggcaggca gagtgccttt
     2821 tgcttggaca agtcctggaa ggaaagaatt tagtttattc agctcctaca agtgctggga
     2881 tgactcttgt ggcagaatta cttattttga agcgggtttt ggaaatgcgg aagaaagctt
     2941 tgtttattct tccctttgtt tctgtggcta aagagaagaa atactacctc cagagtctgt
     3001 ttcaggaagt aggaataaaa gtagacggtt atatgggcag cacctctcca tcaaggcatt
     3061 tctcttcatt ggatattgca gtctgcacaa ttgagagagc caatggtctg atcaatcgcc
     3121 tcatagagga aaataagatg gatctgttag gaatggtggt tgtggatgaa ttacatatgc
     3181 tgggagactc tcaccgaggg tatctgctgg aacttttgct gaccaagatt tgctatatta
     3241 ctcggaaatc agcatcttgt caggcagatc tagccagttc tctgtctaat gctgtgcaaa
     3301 tcgttggcat gagtgctacc cttcctaatt tggagcttgt ggcttcctgg ttgaatgctg
     3361 aactctacca taccgacttt cgccctgtac cgcttttgga gtcagtaaaa gttggaaatt
     3421 ccatatatga ctcttcaatg aaacttgtga gggaatttga gcccatgctt caagtgaagg
     3481 gagatgagga ccatgttgtt agcttatgtt atgagacgat ttgtgataac cattcagtat
     3541 tacttttttg tccatcaaag aaatggtgtg agaagctggc agatatcatt gctcgagagt
     3601 tttataatct acatcatcaa gctgagggat tggtgaaacc ctctgaatgc ccaccagtaa
     3661 ttctggaaca aaaagaactc ctggaagtga tggatcagtt aagacggttg ccttcaggac
     3721 tggactctgt attacagaaa actgtaccat ggggagtagc atttcatcat gcaggtctta
     3781 cttttgagga gagggatatc attgaaggag cctttcgtca aggtctcatt cgagtcttgg
     3841 cggcaacttc tactctttct tctggggtga atttacctgc acgtcgtgtg attattcgaa
     3901 cccctatttt tggtggtcga cctctagata ttcttactta taagcagatg gttggtcgtg
     3961 ctggcaggaa aggagtggac acagtaggcg agagtatctt aatttgtaag aactctgaga
     4021 aatcaaaagg catagctctc cttcagggtt ctctaaagcc tgttcgcagc tgtctgcaaa
     4081 gacgagaagg agaagaagta actggcagca tgatacgagc tattctggag ataatagttg
     4141 gtggagtggc aagtacatca caagatatgc atacttatgc tgcctgcaca tttttggctg
     4201 caagtatgaa agaagggaag caaggaattc agagaaatca agagtctgtt cagcttggag
     4261 cgattgaggc ctgtgtgatg tggctactag aaaatgaatt catccagagt acagaagcca
     4321 gtgatggaac agaaggaaag gtgtatcatc caacacatct tggttcggcc actctttctt
     4381 cttcactttc tccagctgat actttagata tttttgctga cctgcaaaga gcaatgaagg
     4441 gctttgtttt agagaatgat cttcatattc tctatctggt tacacctatg tttgaggatt
     4501 ggactactat tgattggtat cgatttttct gtttatggga gaagttgcca acttcaatga
     4561 aaagggtggc agagctagtg ggagttgaag aggggttctt ggcccgttgt gtgaaaggaa
     4621 aagtagtagc cagaactgag agacagcatc gacaaatggc catccataaa aggtttttca
     4681 ccagtcttgt gctattagat ttaatcagtg aagttccctt aagggaaata aatcagaaat
     4741 atggatgcaa tcgtgggcag attcaatctt tgcaacagtc agctgctgtt tatgcaggga
     4801 tgattacagt attttccaac cgtctgggct ggcacaacat ggaactacta ctttcccaat
     4861 ttcagaagcg tcttacgttt ggcatccaga gggagctgtg tgacctggtt cgggtatcct
     4921 tactaaatgc tcagagagcc agggttctct atgcttctgg ctttcatact gtggcagacc
     4981 ttgctagagc aaatattgtg gaggtggagg tgattctgaa aaatgctgtg cctttcaaaa
     5041 gtgcccggaa ggcagtggat gaggaagagg aagcagttga agaacgtcgc aatatgcgaa
     5101 ctatctgggt gactggcaga aaaggtttaa ctgaaaggga agcagcagcc cttatagtgg
     5161 aagaagccag aatgattctg cagcaggact tagttgaaat gggagtgcaa tggaatccat
     5221 gtgccctgtt acattctagt acatgctcat tgactcatag tgagtccgaa gtaaaggaac
     5281 acacatttat atcccaaact aagagttctt ataaaaaatt aacatcaaag aacaaaagta
     5341 acacaatatt tagtgattct tatattaagc attcaccaaa tatagtgcaa gacttaaata
     5401 aaagtagaga gcatacaagt tcctttaatt gtaatttcca gaatgggaat caagaacatc
     5461 agagatgttc cattttcaga gcaagaaaac gggcctcttt agatataaat aaagagaagc
     5521 caggagcctc tcagaatgag gggaaaacaa gtgataagaa agttgttcag actttttcac
     5581 agaaaacaaa aaaggcacct ttgaatttca attcagaaaa gatgagcaga agttttcgat
     5641 cttggaaacg tagaaagcat ctaaagcgat ctagggacag cagccccctg aaagactctg
     5701 gagcgtgtag aatccattta caaggacaga ctctgtctaa tcctagtctt tgtgaagacc
     5761 cgtttacctt agatgagaag aaaacggaat ttagaaattc agggccattt gctaaaaatg
     5821 tatctttgag tggtaaggaa aaagataata aaacatcatt cccattacaa ataaagcaaa
     5881 attgttcatg gaacataaca ctaactaatg ataattttgt ggagcatatt gtcacaggat
     5941 ctcagagtaa aaatgtgact tgtcaggcca ctagtgtggt tagtgaaaag ggcagaggag
     6001 tagctgttga ggcagaaaaa ataaatgaag tgctgataca aaatggttca aaaaaccaga
     6061 atgtttatat gaaacaccat gacatccatc caattaacca gtacctgcga aagcaatctc
     6121 atgaacagac aagcactatt accaaacaga aaaatataat agagagacaa atgccctgtg
     6181 aagcagtcag tagttacata aatagagact caaatgttac tatcaattgt gaaaggataa
     6241 agcttaatac agaggaaaat aaaccaagtc attttcaggc attaggagat gatataagca
     6301 gaactgtgat acccagtgaa gtacttccat cagctggagc atttagcaaa tcagaaggcc
     6361 agcatgagaa ttttctaaat atttctagac tacaagaaaa aacaggtact tatacaacaa
     6421 acaaaactaa aaataatcat gtttctgact taggtttagt cctctgtgat tttgaagata
     6481 gtttctatct ggatactcag tcagagaaaa taatacaaca gatggcaact gaaaatgcca
     6541 aactaggagc aaaggacacc aacctggcag cagggataat gcagaagagc ttagtccaac
     6601 agaactcaat gaactctttt cagaaggagt gtcacattcc ttttcctgct gaacagcacc
     6661 ctctaggagc gactaagata gatcatttgg accttaagac tgtaggtact atgaaacaaa
     6721 gcagtgattc acatggggtt gatatcctga ctccagaaag cccgattttc cattctccaa
     6781 tactattgga ggaaaatggt ctttttttaa aaaagaatga agtttctgtt actgattcac
     6841 aattaaatag ttttcttcaa ggttatcaaa cacaagaaac tgtgaaacca gttatacttc
     6901 tgattcctca aaagagaact cccactggtg tagaaggaga atgtcttcca gttcctgaaa
     6961 caagtttgaa tatgagtgat agtttactat ttgatagctt cagtgatgac tatctagtaa
     7021 aagaacaatt acctgatatg caaatgaaag aaccccttcc ttcagaagta acatcaaacc
     7081 attttagtga ttctctgtgt ctacaagaag acctaattaa aaaatcaaat gtaaatgaga
     7141 atcaagatac ccaccagcag ttgacttgtt ccaatgatga atctattata ttttcagaaa
     7201 tggattctgt tcagatggtt gaagctttgg acaatgtgga tatatttcct gtccaagaga
     7261 agaatcatac tgtagtatct cctagagcat tagaactaag tgatccagta cttgatgagc
     7321 accaccaagg tgatcaagat ggaggagatc aagatgaaag ggctgaaaaa tcaaaattaa
     7381 ctgggaccag gcaaaatcat tcattcattt ggtcaggggc atcatttgat ctaagtccag
     7441 gactgcaaag gattttagat aaagtatcca gtcctctaga aaatgaaaag ctaaaatcaa
     7501 tgactataaa cttttccagt ttgaatagaa aaaatacaga gttaaatgaa gaacaagaag
     7561 ttatttcaaa cttggagaca aaacaagtgc agggaatttc attttcttct aataatgaag
     7621 taaaaagcaa gattgagatg ctagaaaaca atgccaatca tgatgaaacc tcatccctct
     7681 tacctcgtaa agaaagtaat atagttgatg ataatggtct cattcctcct acacccattc
     7741 caacatctgc ttctaagctg acatttccag ggattcttga aacacctgta aacccttgga
     7801 aaactaataa tgttttacaa cctggtgaaa gttatttatt tggctcacct tcagatatta
     7861 aaaaccacga tttaagtcca gggagtagaa atgggttcaa agacaacagc cctattagtg
     7921 acacaagctt ttcacttcag ttatcacagg atggattaca gttaactcca gcctcaagca
     7981 gttcagaaag tttgtccata attgatgtag caagtgacca aaatcttttc caaacattca
     8041 ttaaggagtg gcggtgcaaa aagcgatttt ccatctcact ggcttgtgaa aagattagaa
     8101 gtttgacatc ttctaaaact gctactattg gcagtaggtt taagcaagct agctcacctc
     8161 aggaaattcc tattagagat gatggatttc ccattaaagg ttgtgatgac accttggtgg
     8221 ttggactggc agtatgctgg ggtggaaggg atgcctatta tttttcactg cagaaggaac
     8281 aaaagcattc tgaaattagt gccagtttgg ttccaccttc tttagatcca agcctgactt
     8341 tgaaagacag gatgtggtac cttcaatctt gcttgcgaaa ggaatctgat aaagaatgtt
     8401 ctgttgtcat ctatgacttc atccagagct ataaaattct tcttctttct tgtggcatct
     8461 ccttggagca aagttatgaa gatcctaagg tggcatgctg gttactagat ccagattctc
     8521 aggagccgac tcttcatagc atagttacca gttttcttcc tcatgagctt ccactcctag
     8581 aagggatgga gaccagccaa gggattcaaa gcctggggct aaatgctggc agtgagcatt
     8641 ctgggcgata cagagcatct gtggagtcca ttctcatctt caactctatg aatcagctca
     8701 actctttgtt gcagaaggaa aaccttcaag atgttttccg taaggtggaa atgccctctc
     8761 agtactgctt ggccttgcta gaactaaatg gaattggctt tagtactgca gaatgtgaaa
     8821 gtcagaaaca tataatgcaa gccaagctgg atgcaattga gacccaggcc tatcaactag
     8881 ctggccacag tttttctttc accagttcag atgacatcgc tgaggtttta tttttggaat
     8941 tgaagttgcc cccaaataga gagatgaaaa accaaggcag caagaaaact ctgggttcta
     9001 ccagaagagg gattgacaat ggacgcaagc taaggctggg aagacagttc agcactagta
     9061 aggacgtttt aaataaatta aaggcattac atcctttacc aggcttgata ttagaatgga
     9121 gaagaatcac taatgctatt accaaagtgg tctttcccct tcagcgggaa aagtgtctta
     9181 atccttttct tggaatggaa agaatctatc ctgtatcaca gtcgcacact gctacaggac
     9241 gaataacctt tacagaacca aatattcaga atgtgccaag agattttgaa atcaaaatgc
     9301 caacactagt aggagaaagc ccaccttctc aagctgtagg caaaggccta cttcccatgg
     9361 gcagaggaaa atataagaag ggtttcagcg tgaatcctag atgccaggca cagatggagg
     9421 agagagctgc agacagagga atgccatttt caattagcat gcgacatgcc tttgtgcctt
     9481 tcccaggtgg ttcaatactg gctgctgccg cctctcagct tgaactgagg atcttggctc
     9541 atttatccca tgatcgtcgt ctcattcaag tgttaaacac tggagctgat gttttcagga
     9601 gcattgcagc agagtggaag atgattgagc cagagtctgt tggggatgat ctgaggcagc
     9661 aggcaaaaca gatttgctat gggatcattt atggaatggg agctaaatct ttgggagagc
     9721 agatgggcat taaagaaaat gatgctgcat gctatattga ctccttcaaa tccagataca
     9781 cagggattaa tcaattcatg acagagacag tgaagaattg taaaagagac ggatttgttc
     9841 agaccatttt gggaaggcgt agatatttgc caggaatcaa agacaacaac ccttatcgta
     9901 aagctcacgc tgagcgtcaa gctatcaaca caatagtcca aggatcagca gctgatattg
     9961 tcaaaatagc cacagttaac attcagaagc aattagagac cttccactca accttcaaat
    10021 cccatggtca tcgagagggt atgctccaaa gtgaccgaac aggattgtca cgaaagagaa
    10081 aactgcaagg gatgttctgc ccaatcagag gaggcttctt catccttcaa ctccatgatg
    10141 aactcctata tgaagtggca gaagaagatg ttgttcaggt agctcagatt gtcaagaatg
    10201 aaatggaaag tgctgtaaaa ctgtctgtga aattgaaagt gaaagtgaaa ataggcgcca
    10261 gctggggaga gctaaaggac tttgatgtgg cggccgctgg aggagactac aaggacgacg
    10321 atgacaagtc ggccgctgga ggatacccct acgacgtgcc cgactacgcc tagggccgcg
    10381 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
    10441 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
    10501 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
    10561 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
    10621 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
    10681 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
    10741 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
    10801 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
    10861 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
    10921 ggacgtggtt ttcctttgaa aaacacgata atacctccgg aatgaccgag tacaagccca
    10981 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    11041 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    11101 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    11161 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    11221 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    11281 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    11341 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    11401 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    11461 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    11521 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta ggtcgacaat caacctctgg
    11581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
    11641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
    11701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    11761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    11821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    11881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    11941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
    12001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    12061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    12121 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
    12181 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
    12241 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
    12301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    12421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
    12481 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
    12541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    12601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    12661 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    12721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    12781 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
    12841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    13021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    13081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    13141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    13201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    13261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    13321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    13381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    13441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    13501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    14041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    14101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    14161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    14221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    14281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    14341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    14401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    14461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    14521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    14581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    14641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    14701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    14761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    14821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    14881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    14941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    15001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    15061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    15121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    15181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    15241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    15301 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    15361 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    15421 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
