LOCUS       sequence-152252-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fusion protein Cas9+BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pLentiCas9-BFP
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..100
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             131..826
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             836..892
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaagcgacc
       61 tgccgccaca aagaaggctg gacaggctaa gaagaagaaa gattacaaag acgatgacga
      121 taagggatcc agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
      181 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
      241 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
      301 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
      361 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
      421 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
      481 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
      541 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
      601 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
      661 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
      721 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
      781 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggat ccggcgcaac
      841 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa
      901 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
      961 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     1021 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctgg
//
