LOCUS       sequence-152256-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 protein with BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pLentiCas9-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
       61 ttcgcaacgg gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaca
      121 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
      181 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
      241 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
      301 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
      361 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
      421 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
      481 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
      541 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
      601 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
      661 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
      721 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
      781 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
      841 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
      901 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
      961 gcgac
//
