LOCUS       sequence-152277-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human PAX3 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hPAX3 (#735)
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
      121 accgccacca tggattacaa ggatgacgac gataagagcc cgggcggatc catgaccacg
      181 ctggccggcg ctgtgcccag gatgatgcgg ccgggcccgg ggcagaacta cccgcgtagc
      241 gggttcccgc tggaagtgtc cactcccctc ggccagggcc gcgtcaacca gctcggcggc
      301 gtttttatca acggcaggcc gctgcccaac cacatccgcc acaagatcgt ggagatggcc
      361 caccacggca tccggccctg cgtcatctcg cgccagctgc gcgtgtccca cggctgcgtc
      421 tccaagatcc tgtgcaggta ccaggagact ggctccatac gtcctggtgc catcggcggc
      481 agcaagccca agcaggtgac aacgcctgac gtggagaaga aaattgagga atacaaaaga
      541 gagaacccgg gcatgttcag ctgggaaatc cgagacaaat tactcaagga cgcggtctgt
      601 gatcgaaaca ccgtgccgtc agtgagttcc atcagccgca tcctgagaag taaattcggg
      661 aaaggtgaag aggaggaggc cgacttggag aggaaggagg cagaggaaag cgagaagaag
      721 gccaaacaca gcatcgacgg catcctgagc gagcgagcct cagcacccca atcagatgaa
      781 ggctctgata ttgactctga accagattta ccactaaaga ggaaacagcg cagaagccga
      841 accaccttca cagcagaaca gctggaggaa ctggagcgtg cttttgagag aactcattac
      901 cctgacat
//
