LOCUS       sequence-152279-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human PAX3 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hPAX3 (#735)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggcttgacc ggacwrtcag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
       61 aagcaggctc cgcggccgcc cccttcaccg ccaccatgga ttacaaggat gacgacgata
      121 agagcccggg cggatccatg accacgctgg ccggcgctgt gcccaggatg atgcggccgg
      181 gcccggggca gaactacccg cgtagcgggt tcccgctgga agtgtccact cccctcggcc
      241 agggccgcgt caaccagctc ggcggcgttt ttatcaacgg caggccgctg cccaaccaca
      301 tccgccacaa gatcgtggag atggcccacc acggcatccg gccctgcgtc atctcgcgcc
      361 agctgcgcgt gtcccacggc tgcgtctcca agatcctgtg caggtaccag gagactggct
      421 ccatacgtcc tggtgccatc ggcggcagca agcccaagca ggtgacaacg cctgacgtgg
      481 agaagaaaat tgaggaatac aaaagagaga acccgggcat gttcagctgg gaaatccgag
      541 acaaattact caaggacgcg gtctgtgatc gaaacaccgt gccgtcagtg agttccatca
      601 gccgcatcct gagaagtaaa ttcgggaaag gtgaagagga ggaggccgac ttggagagga
      661 aggaggcaga ggaaagcgag aagaaggcca aacacagcat cgacggcatc ctgagcgagc
      721 gagcctcagc accccaatca gatgaaggct ctgatattga ctctgaacca gatttaccac
      781 taaagaggaa acagcgcaga agccgaacca ccttcacagc agaacagctg gaggaactgg
      841 agcgtgcttt tgagagaact cattaccctg acat
//
