LOCUS       sequence-152282-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human PAX7 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hPAX7 (#734)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    78..102
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcatagaag acaccgggac cgatccagcc tccgcggccc cgaactagtc cagtgtggtg
       61 gaattctgca gatatcaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
      121 ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc atggcggccc
      181 ttcccggcac ggtaccgaga atgatgcggc cggctccggg gcagaactac ccccgcacgg
      241 gattcccttt ggaagtgtcc accccgcttg gccaaggccg ggtcaatcag ctgggagggg
      301 tcttcatcaa tgggcgaccc ctgcctaacc acatccgcca caagatagtg gagatggccc
      361 accatggcat ccggccctgt gtcatctccc gacagctgcg tgtctcccac ggctgcgtct
      421 ccaagattct ttgccgctac caggagaccg ggtccatccg gcctggggcc atcggcggca
      481 gcaagcccag acaggtggcg actccggatg tagagaaaaa gattgaggag tacaagaggg
      541 aaaacccagg catgttcagc tgggagatcc gggacaggct gctgaaggat gggcactgtg
      601 accgaagcac tgtgccctca gtgagttcga ttagccgcgt gctcagaatc aagttcggga
      661 agaaagagga ggaggatgaa gcggacaaga aggaggacga cggcgaaaag aaggccaaac
      721 acagcatcga cggcatcctg ggcgacaaag ggaaccggct ggacgagggc tcggatgtgg
      781 agtcggaacc tgacctccca ctgaagcgca agcagcgacg cagtcggacc acattcacgg
      841 ccgagcagct gga
//
