LOCUS       sequence-152284-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human PAX7 ORF 
            with a FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-FLAG-hPAX7 (#734)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..123
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggcccsgcc cgtacwgtca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
       61 aaagcaggct ccgcggccgc ccccttcacc gccaccatgg attacaagga tgacgacgat
      121 aagagcccgg gcggatccat ggcggccctt cccggcacgg taccgagaat gatgcggccg
      181 gctccggggc agaactaccc ccgcacggga ttccctttgg aagtgtccac cccgcttggc
      241 caaggccggg tcaatcagct gggaggggtc ttcatcaatg ggcgacccct gcctaaccac
      301 atccgccaca agatagtgga gatggcccac catggcatcc ggccctgtgt catctcccga
      361 cagctgcgtg tctcccacgg ctgcgtctcc aagattcttt gccgctacca ggagaccggg
      421 tccatccggc ctggggccat cggcggcagc aagcccagac aggtggcgac tccggatgta
      481 gagaaaaaga ttgaggagta caagagggaa aacccaggca tgttcagctg ggagatccgg
      541 gacaggctgc tgaaggatgg gcactgtgac cgaagcactg tgccctcagt gagttcgatt
      601 agccgcgtgc tcagaatcaa gttcgggaag aaagaggagg aggatgaagc ggacaagaag
      661 gaggacgacg gcgaaaagaa ggccaaacac agcatcgacg gcatcctggg cgacaaaggg
      721 aaccggctgg acgagggctc ggatgtggag tcggaacctg acctcccact gaagcgcaag
      781 cagcgacgca gtcggaccac attcacggcc gagcagctgg a
//
