LOCUS       sequence-152285-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an 
            IRES-hrGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hMYOG-3xHA-IRES-hrGFP (#324)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             773..853
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggag ctgtatgaga catcccccta
      121 cttctaccag gaaccccgct tctatgatgg ggaaaactac ctgcctgtcc acctccaggg
      181 cttcgaacca ccaggctacg agcggacgga gctcaccctg agccccgagg ccccagggcc
      241 ccttgaggac aaggggctgg ggacccccga gcactgtcca ggccagtgcc tgccgtgggc
      301 gtgtaaggtg tgtaagagga agtcggtgtc cgtggaccgg cggcgggcgg ccacactgag
      361 ggagaagcgc aggctcaaga aggtgaatga ggccttcgag gccctgaaga gaagcaccct
      421 gctcaacccc aaccagcggc tgcccaaggt ggagatcctg cgcagtgcca tccagtacat
      481 cgagcgcctc caggccctgc tcagctccct caaccaggag gagcgtgacc tccgctaccg
      541 gggcgggggc gggccccagc caggggtgcc cagcgaatgc agctctcaca gcgcctcctg
      601 cagtccagag tggggcagtg cactggagtt cagcgccaac ccaggggatc atctgctcac
      661 ggctgaccct acagatgccc acaacctgca ctccctcacc tccatcgtgg acagcatcac
      721 agtggaagat gtgtctgtgg ccttcccaga tgaaaccatg cccaacctcg agtacccata
      781 tgacgttcca gactacgcgt atccgtacga cgttccggat tacgcttacc cttacgatgt
      841 acctgactac gcttaatagc aattcctcga cgactgcata gg
//
