LOCUS       sequence-152287-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an 
            IRES-hrGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hMYOG-3xHA-IRES-hrGFP (#324)
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             698..778
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 cgcggtggcg gccgccacca tggagctgta tgagacatcc ccctacttct accaggaacc
       61 ccgcttctat gatggggaaa actacctgcc tgtccacctc cagggcttcg aaccaccagg
      121 ctacgagcgg acggagctca ccctgagccc cgaggcccca gggccccttg aggacaaggg
      181 gctggggacc cccgagcact gtccaggcca gtgcctgccg tgggcgtgta aggtgtgtaa
      241 gaggaagtcg gtgtccgtgg accggcggcg ggcggccaca ctgagggaga agcgcaggct
      301 caagaaggtg aatgaggcct tcgaggccct gaagagaagc accctgctca accccaacca
      361 gcggctgccc aaggtggaga tcctgcgcag tgccatccag tacatcgagc gcctccaggc
      421 cctgctcagc tccctcaacc aggaggagcg tgacctccgc taccggggcg ggggcgggcc
      481 ccagccaggg gtgcccagcg aatgcagctc tcacagcgcc tcctgcagtc cagagtgggg
      541 cagtgcactg gagttcagcg ccaacccagg ggatcatctg ctcacggctg accctacaga
      601 tgcccacaac ctgcactccc tcacctccat cgtggacagc atcacagtgg aagatgtgtc
      661 tgtggccttc ccagatgaaa ccatgcccaa cctcgagtac ccatatgacg ttccagacta
      721 cgcgtatccg tacgacgttc cggattacgc ttacccttac gatgtacctg actacgctta
      781 atagcaattc ctcgacgact gcatagggtt acccccctct ccctcccccc cccctaacgt
      841 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
      901 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
      961 cattcctagg ggtctttccc ctctcgccaa ggaatgcaag gtctgttgaa tgtcgtgaaa
     1021 gaagcagtct ctgcagctct gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     1081 gtatcccccc ccyacct
//
