LOCUS       sequence-152289-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOG ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYOG-3xHA (#733)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(188..214)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(314..394)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 ggcggaggat aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga
       61 ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatcttg ggtgggttac
      121 tccagactgc cttgggaaaa gcgcctcccc tacccggtag aatttctagt ttaattaatc
      181 attactaagc gtagtctggg acgtcgtatg ggtattcgaa ccgcgggccc tctagactcg
      241 agcggccgcc actgtgctgg atatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      301 cacccttcta ttaagcgtag tcaggtacat cgtaagggta agcgtaatcc ggaacgtcgt
      361 acggatacgc gtagtctgga acgtcatatg ggtactcgag gttgggcatg gtttcatctg
      421 ggaaggccac agacacatct tccactgtga tgctgtccac gatggaggtg agggagtgca
      481 ggttgtgggc atctgtaggg tcagccgtga gcagatgatc ccctgggttg gcgctgaact
      541 ccagtgcact gccccactct ggactgcagg aggcgctgtg agagctgcat tcgctgggca
      601 cccctggctg gggcccgccc ccgccccggt agcggaggtc acgctcctcc tggttgaggg
      661 agctgagcag ggcctggagg cgctcgatgt actggatggc actgcgcagg atctccacct
      721 tgggcagccg ctggttgggg ttgagcaggg tgcttctctt cagggcctcg aaggcctcat
      781 tcaccttctt gagcctgcgc ttctccctca gtgtggccgc ccgccgccgg tccacggaca
      841 ccgacttcct cttacacacc ttacacgccc acggcaggca ctggcctgga cagtgctcgg
      901 g
//
