LOCUS       sequence-152290-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human MYOG ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hMYOG-3xHA (#733)
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             772..852
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(875..899)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             952..978
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggcggggccg acwgtcagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
       61 gcaggctccg cggccgcccc cttcaccgcc accatggagc tgtatgagac atccccctac
      121 ttctaccagg aaccccgctt ctatgatggg gaaaactacc tgcctgtcca cctccagggc
      181 ttcgaaccac caggctacga gcggacggag ctcaccctga gccccgaggc cccagggccc
      241 cttgaggaca aggggctggg gacccccgag cactgtccag gccagtgcct gccgtgggcg
      301 tgtaaggtgt gtaagaggaa gtcggtgtcc gtggaccggc ggcgggcggc cacactgagg
      361 gagaagcgca ggctcaagaa ggtgaatgag gccttcgagg ccctgaagag aagcaccctg
      421 ctcaacccca accagcggct gcccaaggtg gagatcctgc gcagtgccat ccagtacatc
      481 gagcgcctcc aggccctgct cagctccctc aaccaggagg agcgtgacct ccgctaccgg
      541 ggcgggggcg ggccccagcc aggggtgccc agcgaatgca gctctcacag cgcctcctgc
      601 agtccagagt ggggcagtgc actggagttc agcgccaacc caggggatca tctgctcacg
      661 gctgacccta cagatgccca caacctgcac tccctcacct ccatcgtgga cagcatcaca
      721 gtggaagatg tgtctgtggc cttcccagat gaaaccatgc ccaacctcga gtacccatat
      781 gacgttccag actacgcgta tccgtacgac gttccggatt acgcttaccc ttacgatgta
      841 cctgactacg cttaatagaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt
      901 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga atacccatac
      961 gacgtcccag actacgctta gtaatgatta attaaactag aaattctacc gggtagggga
     1021 ggcgcttttc ca
//
