LOCUS       sequence-152291-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral overexpression of human SRF ORF 
            with a 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pINDUCER20-hSRF-3xHA (#757)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(190..216)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    269..293
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(316..396)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 ggcggaagga tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag
       61 gaggggggcg cccgcgggag gcgccaaaac ccggcgcgga ggccagatct tgggtgggtt
      121 actccagact gccttgggaa aagcgcctcc cctacccggt agaatttcta gtttaattaa
      181 tcattactaa gcgtagtctg ggacgtcgta tgggtattcg aaccgcgggc cctctagact
      241 cgagcggccg ccactgtgct ggatatcaac cactttgtac aagaaagctg ggtcggcgcg
      301 cccacccttc tattaagcgt agtcaggtac atcgtaaggg taagcgtaat ccggaacgtc
      361 gtacggatac gcgtagtctg gaacgtcata tgggtactcg agttcactct tgttgctgtg
      421 ggcggtgtcc aggttcacca cctgtagctc ggtgaggttg ctgctgctcc cggcctgctg
      481 ccctatcaca gccatctggt ggagctgaac tgctgaaaca gggatctgca ctgtcccaga
      541 tgatgctgtc aggaacacct gggggacgcc acctggctcc tggacctggc tgtgtgacac
      601 ctgcatggtg gatggtgcct gggagaaggc attcagcacg gtgaggctgc catcacccag
      661 gcccgaggtg ggggcataca tcaccgcatg cgggctaggg tacatcatgt ggccacccac
      721 agttgtgggc acggatgacg tcatgatggt ggcgggcagc gtcactgtcc cgctggagga
      781 ggtctgcgtg aggtctgtgc tgctgtcacg ggagggagac gcttgctgtg gggcctggtg
      841 cacttgaatg gcctgcactg ggacctgctg ggccactgcc
//
