LOCUS       sequence-152304-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human PAX3 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hPAX3 (#395)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatga ccacgctggc cggcgctgtg cccaggatga tgcggccggg
      181 cccggggcag aactacccgc gtagcgggtt cccgctggaa gtgtccactc ccctcggcca
      241 gggccgcgtc aaccagctcg gcggcgtttt tatcaacggc aggccgctgc ccaaccacat
      301 ccgccacaag atcgtggaga tggcccacca cggcatccgg ccctgcgtca tctcgcgcca
      361 gctgcgcgtg tcccacggct gcgtctccaa gatcctgtgc aggtaccagg agactggctc
      421 catacgtcct ggtgccatcg gcggcagcaa gcccaagcag gtgacaacgc ctgacgtgga
      481 gaagaaaatt gaggaataca aaagagagaa cccgggcatg ttcagctggg aaatccgaga
      541 caaattactc aaggacgcgg tctgtgatcg aaacaccgtg ccgtcagtga gttccatcag
      601 ccgcatcctg agaagtaaat tcgggaaagg tgaagaggag gaggccgact tggagaggaa
      661 ggaggcagag gaaagcgaga agaaggccaa acacagcatc gacggcatcc tgagcgagcg
      721 agcctcagca ccccaatcag atgaaggctc tgatattgac tctgaaccag atttaccact
      781 aaagaggaaa cagcgcagaa gccgaaccac cttcacagca gaacagctgg aggaactgga
      841 gcgtgctttt gagagaactc attaccctga catttatact agg
//
