LOCUS       sequence-152312-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Assays activity of transcriptional activators fused to SP Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: gRNA_tdtomato_reporter1_SP-2x
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        139..177
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     174..198
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             221..244
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(402..420)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtggggcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc cgccagtgtg
       61 atggatatct gcagaattcg cccttgcgac gtagggataa cagggtaata gtgtcccctc
      121 caccccacag tggggcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
      181 ttagtgaacc gtcagatcgc ctggagaatt cgccaccatg gactacaagg atgacgacga
      241 taaaacttcc ggtggcggac tgggttccac cgtgagcaag ggcgaggagg tcatcaaaga
      301 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
      361 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      421 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
      481 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
      541 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
      601 ctcctccctg caggacggca cgctgatcta caaggtgaag at
//
