LOCUS       sequence-152313-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recruits p65_hsf1 to compatible gRNAs for transcriptional 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pLEX_307_ms2_p65_hsf1
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..149)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    157..181
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(192..563)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 cacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattgtc
       61 gacttaacgc gttgattgtc gacttaaccg gtactagttc attactacgt agaatcgaga
      121 ccgaggagag ggttagggat aggcttaccg atatcaacca ctttgtacaa gaaagctggg
      181 tttcagctag cggagacagt ggggtccttg gctttgggag gctccgagcc tgtcagcagg
      241 gagatggtgg ggtcctcggc gaagccgtcc ccttcggaga agtaggagcc ctctcccagc
      301 tcaaacagca ccggcaggtc gttgctcccg gtgtccacgg agccggggtc cagcaggaac
      361 agcggctgcg ctgtgtagtg caccagctgc ttccctgaat ccgggctgct gttctctgcc
      421 tcgggaggcc tggggggctc ctggggagac aggagctctt ggatactggc caggctgctg
      481 tcaaggtcag gcaggctcat gtcgggcacg gtcaccgagg ggctgaacag gtccagcagg
      541 gcactggtgt ccacgctgaa gccgcttcca cctcctccct gcccactaga ggaaatctgt
      601 gacagcaggg cactaaagtc catatcagcg atgcttgaga agtcttcatc tccggacagc
      661 ccattaggca ggccgctggt tcccagggga gttggagcgg ggtcgggggg ccgctggctg
      721 ccggtcacca gccgggtaat ggcttcgggg tactccatca gcattggttc ggctgtacta
      781 tgagacatgg acacgccctg attcagcagc tgctgaaact caga
//
