LOCUS       sequence-152316-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of human PAX7 ORF with a N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Alexander lab Muscle Factors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FLAG-hPAX7 (#348)
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccatgg cggcccttcc cggcacggta ccgagaatga tgcggccggc
      181 tccggggcag aactaccccc gcacgggatt ccctttggaa gtgtccaccc cgcttggcca
      241 aggccgggtc aatcagctgg gaggggtctt catcaatggg cgacccctgc ctaaccacat
      301 ccgccacaag atagtggaga tggcccacca tggcatccgg ccctgtgtca tctcccgaca
      361 gctgcgtgtc tcccacggct gcgtctccaa gattctttgc cgctaccagg agaccgggtc
      421 catccggcct ggggccatcg gcggcagcaa gcccagacag gtggcgactc cggatgtaga
      481 gaaaaagatt gaggagtaca agagggaaaa cccaggcatg ttcagctggg agatccggga
      541 caggctgctg aaggatgggc actgtgaccg aagcactgtg ccctcagtga gttcgattag
      601 ccgcgtgctc agaatcaagt tcgggaagaa agaggaggag gatgaagcgg acaagaagga
      661 ggacgacggc gaaaagaagg ccaaacacag catcgacggc atcctgggcg acaaagggaa
      721 ccggctggac gagggctcgg atgtggagtc ggaacctgac ctcccactga agcgcaagca
      781 gcgacgcagt cggaccacat tcacggccga gcagctggag gagctggaga aggcctttga
      841 gaggacccac tacc
//
