LOCUS       sequence-152317-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chim...
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(248..266)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(343..361)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(400..648)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(458..477)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(628..648)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtttaccgta atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
       61 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      121 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgacgaat
      181 actgccattt gtctcgaggt cgagaattca aaaaaaggga agactcccca gtgactgtgg
      241 gagtcgcaca tgggtgatcc tcatgtgctc ccgcaccgac tcggtgccac tttttcaagt
      301 tgataacgga ctagccttat tttaacttgc taggccctgc agacatgggt gatcctcatg
      361 ttggcctagc tctaaaaccg agacgcatta atcgtctccg gtgtttcgtc ctttccacaa
      421 gatatataaa gccaagaaat cgaaatactt tcaagttacg gtaagcatat gatagtccat
      481 tttaaaacat aattttaaaa ctgcaaacta cccaagaaat tattactttc tacgtcacgt
      541 attttgtact aatatctttg tgtttacagt caaattaatt ccaattatct ctctaacagc
      601 cttgtatcgt atatgcaaat atgaaggaat catgggaaat aggccctcgg tgaagggggc
      661 ggccgctcga ggctagtctc gtgatcgata ccgtcgagat ccgttcacta atgataaact
      721 ggatctctgc tgtccctgta ataaacccga aaatt
//
