LOCUS       sequence-152320-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chim...
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(230..248)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(285..303)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(355..373)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(412..660)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(470..489)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(640..660)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
       61 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
      121 taacgcggaa ctccatatat gggctatgaa ctaatgacga atactgccat ttgtctcgag
      181 gtcgagaatt caaaaaaagg gaagactccc cagttactct cggagtcgca catgggtgat
      241 cctcatgtgc tcccgcaccg actcggtgcc acttggccct gcagacatgg gtgatcctca
      301 tgttggccaa gttgataacg gactagcctt attttaactt gctaggccct gcagacatgg
      361 gtgatcctca tgttggccta gctctaaaac cgagacgcat taatcgtctc cggtgtttcg
      421 tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat
      481 atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt
      541 tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat
      601 ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc
      661 ggtgaagggg gcggccgctc gaggctagtc tcgtgatcga taccgtcgag atccgttcac
      721 taatgataaa ctggatctct gctgtccctg taataaaccc gaa
//
