LOCUS       sequence-152321-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(251..269)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(306..324)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(376..394)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(433..681)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(491..510)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(661..681)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcagtttacc gtaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
       61 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      121 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacg
      181 aatactgcca tttgtctcga ggtcgagaat tcaaaaaaag ggaagactcc ccagtgactg
      241 tgggagtcgc acatgggtga tcctcatgtg ctcccgcacc gactcggtgc cacttggccc
      301 tgcagacatg ggtgatcctc atgttggcca agttgataac ggactagcct tattttaact
      361 tgctaggccc tgcagacatg ggtgatcctc atgttggcct agctctaaaa ccgagacgca
      421 ttaatcgtct ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata
      481 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa
      541 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
      601 agtcaaatta attccaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
      661 aatcatggga aataggccct cggtgaaggg ggcggccgct cgaggctagt ctcgtgatcg
      721 ataccgtcga gatccgttca ctaatgataa actggatctc tgctgtccct gtaataaacc
      781 cgaa
//
