LOCUS       sequence-152323-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..46)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(286..305)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             445..696
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     633..654
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(765..785)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
       61 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      121 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
      181 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      241 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      301 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      361 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgccatc
      421 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
      481 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      541 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
      601 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      661 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcgtctggaa caatcaacct
      721 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      781 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcc
//
